Project description:Cartilage endplate-derived stem cells (CESCs) with chondro-osteogenic differentiation capacity may be responsible for the balance of chondrification and ossification in cartilage endplate (CEP). CEP is an avascular and hypoxic tissue, and hypoxia could influence the fate of CESCs. We used high-throughput scanning to identify differentially expressed genes (DEGs) and alternatively spliced genes (ASGs) of CESCs under hypoxia compared to those under normoxia. Human cartilage endplate-derived stem cells (CESCs) were cultured under normoxia and hypoxia for 21 days respectively.
Project description:Cartilage endplate-derived stem cells (CESCs) with chondro-osteogenic differentiation capacity may be responsible for the balance of chondrification and ossification in cartilage endplate (CEP). CEP is an avascular and hypoxic tissue, and hypoxia could inhibit the osteogenic differentiation of CESCs. We used high-throughput scanning to identify differentially expressed genes (DEGs) and alternatively spliced genes (ASGs) during osteogenic differentiation of CESCs under hypoxia compared to those induced under normoxia. Human cartilage endplate-derived stem cells (CESCs) were treated with osteogenic differentiation medium under normoxia and hypoxia for 21 days respectively.
Project description:Cartilage endplate-derived stem cells (CESCs) with chondro-osteogenic differentiation capacity may be responsible for the balance of chondrification and ossification in cartilage endplate (CEP). CEP is an avascular and hypoxic tissue, and hypoxia could influence the fate of CESCs. We used high-throughput scanning to identify differentially expressed genes (DEGs) and alternatively spliced genes (ASGs) of CESCs under hypoxia compared to those under normoxia.
Project description:Cartilage endplate-derived stem cells (CESCs) with chondro-osteogenic differentiation capacity may be responsible for the balance of chondrification and ossification in cartilage endplate (CEP). CEP is an avascular and hypoxic tissue, and hypoxia could inhibit the osteogenic differentiation of CESCs. We used high-throughput scanning to identify differentially expressed genes (DEGs) and alternatively spliced genes (ASGs) during osteogenic differentiation of CESCs under hypoxia compared to those induced under normoxia.
Project description:To identify the effects of hypoxia on stem cell populations, we subjected human mesenchymal stem cells to a pO2 of 4 mmHg and analyzed global gene expression and alternative splicing (AS) by genome-exon microarray. Hypoxia induced gene expression in human mesenchymal stem cells was measured at 24 hours after exposure to 0.5% oxygen or normal 21% oxygen. Three independent experiments were performed per condition (Hypoxia or Normoxia).
Project description:Multipotent bone marrow-derived mesenchymal stem cells (MSCs) represent a promising cell source for autologous transplantation in many human diseases. To better realize their in vivo therapeutic potential, hypoxia preconditioning has become a novel strategy to improve the survival, migration, and angiogenic capability of MSCs. To elucidate the molecular mechanisms underlying the beneficial effect of hypoxia preconditioning on MSCs, we respectively used standard expression microarray and exon microarray to investigate the global profiling of gene expression and alternative splicing in hypoxia preconditioned-MSCs, and the detection sensitivity of differential gene expression using exon microarray and standard expression microarray was compared. A total of 414 genes were identified as significantly differentially expressed using standard expression microarray, while only 72 genes were identified as significantly differentially expressed using exon microarray, suggesting that standard expression microarray should be preferred if exclusively to detect changes in gene level. Our finding generated a global profiling of gene expression and alternative splicing which may be responsible for enhanced therapeutic effect of the hypoxia preconditioned-hMSCs. Our work provides a general framework for the systematic study of stem cell biology under clinically relevant pathophysiologic conditions Gene expression/alternative splicing in human mesenchymal stem cells (hMSCs) isolated from human bone marrow were measured after exposure to 0.5% O2 or 21% O2 for 24 hours using gene expression array/exon array. Three independent experiments were performed using different donors for each experiment for both gene expression array analysis and exon array analysis.
Project description:Gene Expression microarrays were used to evaluate gene expression differences between induced pluripotent stem cells (iPSCs) cultured under hypoxia and normoxia conditions
Project description:Heart fibroblasts from wildtype mice and Siah2-/- knockout mice were isolated and cultured. The cells were either left untreated or incubated for 6 hs under hypoxic conditions. One experiment consists of wildtype cells (normoxia/hypoxia) and Siah2 knockout cells (normoxia/hypoxia) = 4 samples. To allow statistical analysis of the data set the experiment was repeated once under identical conditions.
Project description:Low back pain (LBP) is one of the most prevalent conditions which need medical advice and result in chronic disabilities. Degenerative disc disease (DDD) is a common reason for LBP. A lot of researchers think that CEP degeneration play critical roles in the initiation and development of DDD. In recent years, researchers have put interests on cell-based therapies for regenerating disc structure and function. Our research team has isolated cartilage endplate-derived stem cells (CESCs) and validated their chondrogenic and osteogenic differentiation ability. Enhanced chondrogenic differentiation and inhibited osteogenic differentiation of CESCs may retard CEP calcification and restore the nutrition supply, possibly regenerating the degenerated discs. We used Affymetrix Human Transcriptome Array 2.0 to study the global gene expression profilling and alternative splicing events during the chondrogenic and osteogenic differentiation of cartilage endplate-derived stem cells. The cartilage endplate-derived stem cells(CESCs) were induced to undergo chondrogenic(CD) and osteogenic differentiation(OD). Both undifferentiated and differentiated CESCs were sent for RNA extraction and hybridization on Affymetrix microarrays. A comparative analysis was done between the undifferentiated and differentiated samples.
