Project description:We report the application of next generation RNA sequencing to analyze the transcriptional response of Drosophila adult flies to infection by the insect pathogenic nematodes Heterorhabditis bacteriophora and their mutualistic bacteria Photorhabdus luminescens, either separately or together. We find that Heterorhabditis and Photorhabdus differentially modulate a large number of genes, many of which participate in metabolic functions, stress responses, repression of gene transcription and neuronal activities. We have also identified Drosophila genes with potential role in nematode recognition and others with putative anti-nematode properties. These findings generate novel insights into how the host immune function is shaped to respond against nematode parasites and their associated bacteria. Transcriptional profiles of Drosophila wild-type adult flies infected with Heterorhabditis bacteriophora carrying or lacking Photorhabdus or the bacteria alone were generated at 12 and 30 hours post infection using Illumina deep sequencing technology.

2015-09-12 | E-GEOD-61466 | biostudies-arrayexpress

Photorhabdus luminescens strain:DSM 22397 | isolate:DSM 22397

Project description:Photorhabdus luminescens hainanensis DSM 22397 genome sequencing

| PRJNA368470 | ENA

Photorhabdus temperata strain:DSM 22387 | isolate:DSM 22387

Project description:Photorhabdus temperata tasmaniensis DSM 22387 genome sequencing

| PRJNA331494 | ENA

Photorhabdus temperata strain:DSM 14550 | isolate:DSM 14550

Project description:Photorhabdus temperata temperata DSM 14550 genome sequencing

| PRJNA368476 | ENA

Photorhabdus luminescens strain:DSM 15139 | isolate:DSM 15139

Project description:Photorhabdus luminescens laumondii DSM 15139 genome sequencing