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Omics score: 1000

Aspergillus flavus strain:TJW149.27

ABSTRACT: Aspergillus flavus 3357 delta-ku70 T0-2 Resequencing genome sequencing

PROVIDER: PRJNA333839 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR4142458_1.fastq.gz	Fastqsanger.gz
	SRR4142458_2.fastq.gz	Fastqsanger.gz

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Aspergillus flavus strain:TJW149.27

Project description:Aspergillus flavus 3357 delta-ku70 T0-1 Resequencing genome sequencing

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Genome Sequence of Aspergillus flavus NRRL 3357, a Strain That Causes Aflatoxin Contamination of Food and Feed.

Project description:Aflatoxin contamination of food and livestock feed results in significant annual crop losses internationally. Aspergillus flavus is the major fungus responsible for this loss. Additionally, A. flavus is the second leading cause of aspergillosis in immunocompromised human patients. Here, we report the genome sequence of strain NRRL 3357.

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A Novel Site-Specific Integration System for Genetic Modification of Aspergillus flavus.

Project description:Aspergillus flavus is a fungus that produces aflatoxin B1, one of the most carcinogenic secondary metabolites. Understanding the regulation mechanism of aflatoxin biosynthesis in this fungus requires precise methods for genomic integration of mutant alleles. To avoid the disadvantage of DNA integration into the genome by non-homologous or ectopic recombination, we developed a novel strategy for site-specific integration of foreign DNA by using a carboxin-resistant sdh2R allele (His 249 Leu). Our results demonstrated that the transformants were generated with a high efficiency (>96%) of correct integration into the sdh2-lcus of the genome of A. flavus NRRL 3357. The advantage of this method is that introduction of the eGFP expression cassette into the sdh2-locus had little effect on fungal growth and virulence while also being rapid and efficient. This system will be a valuable tool for genetic manipulation in A. flavus To the best of our knowledge, this is the first report on the efficient site-specific integration at the sdh2-locus in the genome of Aspergillus.

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Comparative Chemistry of Aspergillus oryzae (RIB40) and A. flavus (NRRL 3357).

Project description:Aspergillus oryzae and A. flavus are important species in industrial biotechnology and food safety and have been some of the first aspergilli to be fully genome sequenced. Bioinformatic analysis has revealed 99.5% gene homology between the two species pointing towards a large coherence in the secondary metabolite production. In this study we report on the first comparison of secondary metabolite production between the full genome sequenced strains of A. oryzae (RIB40) and A. flavus (NRRL 3357). Surprisingly, the overall chemical profiles of the two strains were mostly very different across 15 growth conditions. Contrary to previous studies we found the aflatrem precursor 13-desoxypaxilline to be a major metabolite from A. oryzae under certain growth conditions. For the first time, we additionally report A. oryzae to produce parasiticolide A and two new analogues hereof, along with four new alkaloids related to the A. flavus metabolites ditryptophenalines and miyakamides. Generally the secondary metabolite capability of A. oryzae presents several novel end products likely to result from the domestication process from A. flavus.

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Project description:Aspergillus flavus 3357 wt T0-2 Resequencing genome sequencing

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Project description:Aspergillus flavus 3357 wt T0-1 Resequencing genome sequencing

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