Project description:Mrakia psychrophlila is a psychrophilic fungus widely spread all over the world. The cold adaptation mechanism of Mrakia psychrophila is unknown. Using RNA sequencing, differentially expressed genes of Mrakia psychrophila cultured at 4, 12 and 20 degree centigrade are identified. The result may be benefit for understanding cold adaptation of microorganisms.
Project description:Corynebacterium glutamicum strain ATCC 21831 is a producer of L-arginine that was created by random mutagenesis. It is resistant to the arginine structural analogue canavanine. In order to identify potential bottlenecks in the biosynthetic pathway that leads to this industrially important amino acid, relative metabolite abundances of biosynthetic intermediates were determined in comparison to the type strain ATCC 13032. An extract of U13C-labeled biomass was used as internal standard, to correct for different ionization efficiencies. Metabolites were identified using the ALLocator web platform.