Project description:Transcription activator-like effectors (TALEs) from Xanthomonas activate host susceptibility genes to promote disease. We report here the tomato transcriptome changes after inoculation with Xanthomonas strains delivering Brg11*, a TALE-like effector from Ralstonia solanacearum or delivering the Brg11-derivative lacking the DNA binding domain as a control. After diffenential gene expression analysis we identified 117 genes were up-regulated and 8 genes were down-regulated by Brg11. This provides us a candidate gene list facilitating identification of Brg11 direct target genes in tomato.
Project description:This SuperSeries is composed of the following subset Series: GSE9640: Transcriptome Profiling of Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola on two different medias GSE9643: Transcriptome Profiling of Xanthomonas oryzae pv. oryzae knockout mutants at different hybridization conditions and PMTs Keywords: SuperSeries Refer to individual Series
Project description:African Xanthomonas oryzae pv. oryzae strains seem most closely related to and share several genetic features with pathovar oryzicola despite causing symptoms of bacterial leaf blight. The ability of most Xanthomonas plant pathogenic bacteria to infect their host relies on the action of a specific family of type III effectors called the TAL effectors. These microbial transcription factors are injected into the plant and manipulate the host transcriptome upon binding to the promoters of plant genes. The genes whose induction is of benefit to the pathogen are called susceptibility genes. RNA profiling experiments in rice using the Malian Xoo strain MAI1 and in silico prediction of TAL effector binding sites were carried out to identify candidate targets of TalB, revealing OsTFX1, a bZIP transcription factor previously identified as a bacterial blight S gene, and OsERF#123, which encodes a subgroup IXc AP2/ERF transcription factor.