Project description:Whole Genome Metabolism of "Macrococcus caseolyticus (strain JCSC5402)"
This is a whole genome metabolism model of Macrococcus caseolyticus (strain JCSC5402).
This model has been automatically generated by the SuBliMinaL Toolbox
and libAnnotationSBML using information coming from from KEGG (release 66, April 2013, accessed via the resource's web services interface) and, where relevant, augmented with metabolic pathway information extracted from MetaCyc (version 17.0, March 2013).
This model has been produced by the path2models
project and is currently hosted on BioModels Database
and identified by: BMID000000140841
Other models with the same genus include BMID000000005930 BMID000000005931 BMID000000005932 BMID000000005933 BMID000000005934 BMID000000005935 BMID000000005936 BMID000000005937 BMID000000005938 BMID000000005939 BMID000000005940 BMID000000005941 BMID000000005942 BMID000000005943 BMID000000051918 BMID000000051919 BMID000000051920 BMID000000051921 BMID000000051922 BMID000000051923 BMID000000051924 BMID000000051925 BMID000000051926 BMID000000051927 BMID000000051928 BMID000000051929 BMID000000051930 BMID000000051931 BMID000000051932 BMID000000051933 BMID000000051934 BMID000000051935 BMID000000051936 BMID000000051937 BMID000000051938 BMID000000051939 BMID000000051940 BMID000000051941 BMID000000051942 BMID000000051943 BMID000000051944 BMID000000051945 BMID000000051946 BMID000000051947 BMID000000051948 BMID000000051949 BMID000000051950 BMID000000051951 BMID000000051952 BMID000000051953 BMID000000051954 BMID000000051955 BMID000000051956 BMID000000051957 BMID000000051958 BMID000000051959 BMID000000051960 BMID000000051961 BMID000000051962 BMID000000051963 BMID000000051964 BMID000000051965 BMID000000051966 BMID000000051967 BMID000000051968 BMID000000051969 BMID000000051970 BMID000000051971 BMID000000051972 BMID000000051973 BMID000000051974 BMID000000051975 BMID000000051976 BMID000000051977 BMID000000051978 BMID000000051979 BMID000000051980 BMID000000051981 BMID000000051982 BMID000000051983 BMID000000051984 BMID000000051985 BMID000000051986 .
To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication
for more information.
Project description:The features of Mycoplasma in human organ such lung and urinary tract are enigmatic. Here, the role of M. hominis in regard to biofilm formation of uropathogenic Escherichia coli (UPEC) strain CFT073 was investigated. Although M. hominis were inferred to not impact on UPEC bacterial fitness including growth and productions of signaling molecules as autoinducer-2 (AI-2) and indole, we found that the presence of M. hominis dramatically decreased biofilm formation of UPEC CFT073 as well as slightly repressed attachment and cytotoxicity of that. Importantly, this activity was observed on UPEC strain specifically, not enterohemorrhagic E. coli (EHEC) strain that exists on intestine. Whole-transcriptome profiling and quantitative real-time polymerase chain reaction (qRT-PCR) analysis revealed PhoPQ system and anti-termination protein (encoded by ybcQ) participates on the reduction of biofilm formation by M. hominis (corroborated by qRT-PCR). Furthermore, collaborating with previous report that toxin-antitoxin (TA) system involved in biofilm formation, M. hominis increased on the transcriptions of toxin genes including hha (toxin gene in Hha-TomB TA system) and pasT (toxin part in PasT-PasI TA system). Hence, we propose that one possible role of M. hominis is to influence bacterial biofilm formation in urinary tract. Only fourteen genes were induced (2.5-fold) by the presence of M. hominis in Uropathogenic Escherichia coli (UPEC) biofilm cells. Among upregulated genes, ybcQ (encodes anti-termination protein Q homolog) and phoP/phoQ (encode DNA-binding response regulators in two-component regulatory system), were induced by the presence of M. hominis. Two-condition experiment, UPEC CFT073 alone vs. UPEC CFT073 with Mycoplasma hominis PG21 (10^5 ccu/ml). For preparing the total RNA, UPEC CFT073 cells were grown at 37°C in biofilm cells on glass wool with or without M. hominis for 24 h.