Project description:Small RNA sequencing on trophoblast debris samples was employed to profile the small RNA contents in either normotensive or preeclamptic trophoblast debris. We have identified 1278 miRNAs and 2646 non-miRNA small RNA fragments across all trophoblast debris samples. Differential expression analysis was executed by iSRAP small RNA sequencing analysis pipeline and we identified 16 miRNAs, 5 tRNA fragments from 3 different tRNAs, 13 snRNA fragments and 85 rRNA fragments differentially contained between preeclamptic and normotensive trophoblast debris
Project description:The transcriptomic profile of human microvascular endothelial cells after exposure to preeclamptic or normotensive third trimester trophoblast debris
Project description:Placental Tissue Samples from 36 women (17 normotensive women, denoted with a P, and 19 preeclamptic women, denoted with a Q) were analyzed for differenital methylation Preeclamptic womene were compared direclty to normotensive women controlling for gestational age, race, maternal age, and baby sex 36 samples were analyzed: 17 normotensive, 19 preeclamptic
Project description:Placental Tissue Samples from 36 women (17 normotensive women, denoted with a P, and 19 preeclamptic women, denoted with a Q) were analyzed for differenital methylation Preeclamptic womene were compared direclty to normotensive women controlling for gestational age, race, maternal age, and baby sex
Project description:Placental Tissue Samples from 36 women (17 normotensive women, denoted with a P, and 19 preeclamptic women, denoted with a Q) were analyzed for differenital methylation Preeclamptic womene were compared direclty to normotensive women controlling for gestational age, race, maternal age, and baby sex
Project description:Placental Tissue Samples from 36 women (17 normotensive women, denoted with a P, and 19 preeclamptic women, denoted with a Q) were analyzed for differenital methylation Preeclamptic womene were compared direclty to normotensive women controlling for gestational age, race, maternal age, and baby sex 36 samples were analyzed, 18 in each group
Project description:RNA-sequencing (RNA-Seq) protocols and bioinformatic pipelines are designed to streamline downstream analyses on sequences believed to be the most important. Here, we have challenged this dogma by preserving ribosomal RNA (rRNA) in our samples and by lowering the minimal RNA size window of our small RNA-Seq analyses to 8 nt
