Drosophila melanogaster
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA340284 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Transcriptional profiling of Drosophila melanogaster 2nd chromosome substitution lines; Background chromosomes are identical across lines; 2nd chromosomes are different across line and can be homozygous or heterozygous within each line Keywords: Natural variation
2010-05-06 | GSE12191 | GEO
Project description:In D. melanogaster, the subdivision of dorsal ectoderm into amnioserosa (AS) and dorsal epidermis is controlled by the combined actions of two morphogens of the TGF-β family, Decapentaplegic (Dpp) and Screw (Scw). They form an extracellular gradient with peak levels of Dpp/Scw signalling in the dorsal-most region of the embryo. Dpp/Scw signalling is transduced to the nucleus by a complex containing the phosphorylated Smad transcription factor, pMad and the co-Smad, Medea, which are responsible for the transcriptional activation of a number of targets in specific dorsal territories, including zerknullt (zen) gen. zen encodes a homeobox transcription factor, which is expressed for a brief period of time in the early developing embryo and is required for all aspects of AS formation, including the initial cell shape changes that drive the morphogenesis of the AS epithelium. However, the number and nature of Zen-target genes involved in AS differentiation subsequent to its specification are currently unknown. The only known target genes of Zen are the so called u-shaped genes; they encode transcription factors that control the maintenance of AS after Zen activity has faded away, so they are not informative for elucidating the specification of morphological properties on a cellular level. In this project, we attempted to identify Zen-target genes required for AS differentiation and/or maintenance. Methods: D. melanogaster flies were grown at 22 ºC on standard cornmeal, molasses, agar, yeast medium. Staged embryos were obtained as described in Zuñiga et al. (2009). Fly strain was zen7, a null allele balanced over TM3, ftz-lacZ (Rushlow et al., 1987). zen7 homozygous embryos were selected using a genotyping procedure described by Ghanim and White (2006). In our case, both the absence of lacZ specific band together with the presence of a control band (non-coding region of gene Dtg) were indicators of homozygous lethal embryos. For RNA extractions, extracts of homozygous (zen7) and heterozygous (zen7/TM3, ftz-lacZ) embryos at stage 5 or stage 6-7 of embryogenesis , previously preserved in RNAWIZ reagent (N = 100) were pooled, then samples were carefully homogenized in a 1.5 mL Eppendorf tube on ice with the aid of RNAse-free polypropylene pellet pestle. After homogenization, RNA extraction was performed using standard protocols. RNA was quantified using Qubit® RNA HS Assay Kit (Thermo Fisher) and the integrity was assayed in a Tape station 2200 (Agilent Technologies). Samples were treated with Turbo DNA-free DNase (Ambion) to remove contaminating DNA. Two biological replicates of RNA from of homozygous and heterozygous embryos were sequenced using XXX and the sequence reads that passed quality filters were analyzed using XXX
2016-08-27 | GSE86114 | GEO
Project description:Gene expression timecourse: SoxN homozygous mutant embryos vs SoxN heterozygous mutant embryos.
| PRJNA205169 | ENA
Project description:Transcriptional profiling of Drosophila melanogaster 2nd chromosome substitution lines; Background chromosomes are identical across lines; 2nd chromosomes are different across line and can be homozygous or heterozygous within each line Keywords: Natural variation Loop design with 8 genotypes
2010-05-06 | E-GEOD-12191 | biostudies-arrayexpress
Project description:Protein expression levels of Drosophila melanogaster embryos were monitored by SWATH-MS after heat-shock treatments.
2016-12-23 | PXD004753 | Pride
Project description:Expression profiling pooled Drosophila melanogaster heterozygous for deletions on Chromosome 2L
| PRJNA261333 | ENA
Project description:Transcriptional profiling of Indy long lived flies and controls over the course of their entire lifespan. Mutations in the Indy gene extend life span in Drosophila melanogaster. This study investigates the changes in gene expression over time in Indy206 flies heterozygous over Canton-S (Indy206/CS) and compares them to genetically matched heterozygous controls (2216/CS). Samples from both fly strains were collected at age: 5, 10, 20, 30, 40, 50, 70 and 80.
2009-03-08 | GSE13872 | GEO
Project description:GAF-depleted Drosophila melanogaster embryos
2023-06-27 | GSE228614 | GEO
Project description:ELAV ChIP-seq in Drosophila melanogaster embryos
| PRJNA267499 | ENA
Project description:Hi-C of early Drosophila melanogaster embryos
| PRJNA391471 | ENA