Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Human polar body transfer eliminates the transmission of inherited mitochondrial DNA variants

ABSTRACT: Human polar body transfer eliminates the transmission of inherited mitochondrial DNA variants

PROVIDER: PRJNA342524 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Human polar body transfer eliminates the transmission of inherited mitochondrial DNA variants [methylation]

Project description:Although polar body transfer (PBT) has the potential to prevent the transmission of inherited mitochondrial DNA (mtDNA) diseases, the PBT technique is still at an early stage, as no human data are publicly available for PBT. Here, we investigated the comparative values of first and second PBT (PB1T, PB2T), spindle-chromosome and pronuclear transfer (ST, PNT), modified ST and PNT (mST and mPNT) to explore the efficiency and safety of these approaches. A comparative analysis confirmed that PB1T, mST, PB2T and mPNT could be used to donate mtDNA without resulting in significant heteroplasmy or alterations in the methylation profile and gene expression. Importantly, PB1T produced reconstructed embryos and embryonic stem cells (ESCs) in every generation with undetectable donor mtDNA. However, donor mtDNA seems to have a tendency to be amplified in generations of mPNT-ESCs with up to 3% heteroplasmy. These results suggest that PB1T holds great potential in eliminating mtDNA variants.

2016-09-11 | GSE86648 | GEO

Human polar body transfer eliminates the transmission of inherited mitochondrial DNA variants [mRNA]

2016-09-11 | GSE86570 | GEO

Human polar body transfer eliminates the transmission of inherited mitochondrial DNA variants

Project description:This SuperSeries is composed of the SubSeries listed below.

2016-09-11 | GSE86650 | GEO

DNA methylation data from ESCs derived by polar body transfer reconstructed embryos (PBTESCs)

Project description:Here we report the derivation of human ESCs from the polar body thansfer reconstructed embryos. We choose three cell lines from all the cell lines and compare the DNA methylation state. We use human methylation chip to compare genomic DNA methylation level among three PB1 transfer human ES cell lines and three PB2 transfer human ES cell lines.

2018-03-04 | GSE95761 | GEO

mRNA expression data from ESCs derived by polar body transfer reconstructed embryos (PBTESCs)

Project description:Here we report the derivation of human PBTESCs from polar body transfer resconstructed embryos. We used RNA-seq to compare the gene expression levels among human parthenogenetic haploid ESCs (hPGES)、normal human ESCs (H9) and human forskin fibroblasts and identified that these cells express conventional ESCs pluripotent markers and most maternally imprinted genes were down-regulated.

2018-03-04 | GSE95706 | GEO

Comparison of the human oocyte to its sister polar body

Project description:Clinicians need additional metrics for predicting quality of human oocytes for IVF procedures. Human polar bodies reflect the oocyte transcript profile. Quantitation of polar body mRNAs could allow for both oocyte ranking and embryo preferences in IVF applications. The transcriptome of a polar body has never been reported, in any organism. Eight total samples. There are 2 biological replicates of the following four conditions: pooled oocytes and their sister polar bodies and a single oocyte and its sister polar body.

2011-10-12 | E-GEOD-32689 | biostudies-arrayexpress

Comparison of the human oocyte to its sister polar body

2011-10-12 | GSE32689 | GEO

The second polar body contributes to the fate asymmetry in the mouse embryo

Project description:The second polar body (PB2) of mouse embryo may contains some crutial factors that control fate asymmetry. This study is aimed at discovering differentially expressed genes in PB2 compared with zygote.

2021-12-27 | GSE182918 | GEO

Daphnia magna exposed to narcotics and polar narcotics

Project description:This SuperSeries is composed of the following subset Series: GSE29854: Daphnia magna exposed to narcotics and polar narcotics - aniline GSE29856: Daphnia magna exposed to narcotics and polar narcotics - 4-chloroaniline GSE29857: Daphnia magna exposed to narcotics and polar narcotics - 3,5-dichloroaniline GSE29858: Daphnia magna exposed to narcotics and polar narcotics - 2,3,4-trichloroaniline GSE29862: Daphnia magna exposed to narcotics and polar narcotics - ethanol GSE29864: Daphnia magna exposed to narcotics and polar narcotics - isopropanol GSE29867: Daphnia magna exposed to narcotics and polar narcotics - methanol Refer to individual Series

2012-02-01 | E-GEOD-29993 | biostudies-arrayexpress

Acquired somatic variants in inherited myeloid malignancies

Project description:Acquired somatic variants in inherited myeloid malignancies

| PRJEB49554 | ENA