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Gene expression changes in Tulasnella calospora- Serapias vomeracea mycorrhizal protocorms at stage P2 compared to Tulasnella calospora free-living mycelium

ABSTRACT: Gene expression changes in Tulasnella calospora- Serapias vomeracea mycorrhizal protocorms at stage P2 compared to Tulasnella calospora free-living mycelium

OTHER RELATED OMICS DATASETS IN: GSE87120GSE86968

PROVIDER: PRJNA343111 | ENA |

REPOSITORIES: ENA

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Gene expression changes in Tulasnella calospora - Serapias vomeracea mycorrhizal protocorms at stage P2 compared to asymbiotic, achlorophyllous Serapias vomeracea protocorms

Project description:Illumina HiSeq technology was used to generate mRNA profiles from Serapias vomeracea-Tulasnella calospora mycorrhizal protocorms compared to asymbiotic protocorms . Protocorms were harvested at stage P2 and used for RNA extraction. Reads of 150bp were generated and aligned to a de novo transcriptome assembly from Serapias vomeracea using CLC Genomics Workbench 9.

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Gene expression changes in Tulasnella calospora- Serapias vomeracea mycorrhizal protocorms at stage P2 compared to Tulasnella calospora free-living mycelium

Project description:Illumina HiSeq technology was used to generate mRNA profiles from Tulasnella calospora mycorrhizal protocorms at stage P2 compared to free-living mycelium grown on Modified Melin-Norkrans medium with two different N sources, ammonium (AL13M) and glutamine (AL1 G) . Protocorms were harvested at stage P2 and mycelium after 15 days and used for RNA extraction. Reads of 150bp were generated and aligned to Tulasnella calospora transcripts (http://genome.jgi-psf.org/Tulca1) using CLC Genomics Workbench 9.

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Gene expression changes in Tulasnella calospora- Serapias vomeracea mycorrhizal protocorms compared to Tulasnella calospora free-living mycelium

Project description:Illumina HiSeq technology was used to generate mRNA profiles from Tulasnella calospora mycorrhizal protocorms compared to free-living mycelium . Protocorms and control mycelium were harvested after 30 days and used for RNA extraction. Reads of 2X100bp were generated and aligned to Tulasnella calospora transcripts (http://genome.jgi-psf.org/Tulca1) using CLC Genomics Workbench 6. mRNA profiles from Tulasnella calospora mycorrhizal protocorms and free-living mycelium were generated by paired-end (2x100bp) Illumina HiSeq2000 sequencing. Three biological replicates were sequenced for mycorrhizal and mycelium samples.

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