Project description:P. savastanoi pv. phaseolicola isolate R5 was cultured in 10 mL Luria broth with 400 uM genistein or without (but with 50 uL DMSO) at 28 C at 200 rpm on a shaking platform. Protein was prepared from 3 replicates, digested, and labeled with TMT10. Tags 126, 127N, and 128N were assigned to control replicates, and 129N, 130N and 131 were assigned to genistein-treated replicates. 12 fractions were analyzed on an Orbitrap Eclipse.
Project description:In order to obtain a global view about the strategies used by phytopathogenic bacteria, in response to physiologically relevant temperature changes. We used the DNA microarray technology to compare gene expression profile in the model bacterial pathogen P. syringae pv. phaseolicola NPS3121 grown at 18 M-BM-:C and 28M-BM-:C. To carry out this study, we used this DNA microarray of P. syringae pv. phaseolicola NPS3121. Each microarray experiment was repeated six times; two technical replicates with the same RNA samples and three biological replicates using RNA isolated from a different culture.