Project description:Whole transcriptome RNA sequencing in primary fibroblasts of outbred Peromyscus maniculatus was generated to explore the degree of coordination in gene expression.
Project description:Whole transcriptome RNA sequencing in brain tissue was generated to explore differences between young and old animals of two closely related species of deer mice (genus Peromyscus) that reportedly differ in their lifespans: P. leucopus that lives for up to 8 years and P. maniculatus that exhibits a lifespan of about 4 years.
Project description:ABSTRACT: The cactus mouse (Peromyscus eremicus) is a desert-specialized rodent that experiences both chronic and acute dehydration in the Southwestern United States. Our previous research has generated substantial transcriptomic data on P. eremicus kidneys, testes, epididymis, and vas deferens in individuals exposed to hydrated and dehydrated conditions; however, the study described here is the first to describe a seminal vesicle proteome for this species. We have produced a seminal vesicle proteome from P. eremicus with free access to water and mice that were acutely dehydrated to generate a dataset that is comprehensive for both alternative water-availability states experienced by this species. We have also provided gene ontologies for this proteome using PANTHER. This proteome will provide a crucial resource for future studies characterizing the genetic and proteomic responses of reproductive tissues to drought in this rodent. Furthermore, an enhanced understanding of survival and reproductive responses (and adaptations) to dehydration is particularly relevant to clinical work aiming to minimize adverse human impacts as climate change continues to increase the incidence of drought.
