Macaca fascicularis
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ABSTRACT:
PROVIDER: PRJNA345224 | ENA |
REPOSITORIES: ENA
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Project description:Generation of Cynomolgus Monkey Chimeric Fetuses Using Embryonic Stem cells
| PRJNA286231 | ENA
Project description:The long-tailed macaque, also referred to as cynomolgus monkey (Macaca fascicularis), is one of the most important non-human primate animal models in basic and applied biomedical research. To improve the predictive power of primate experiments for humans, we determined the genome sequence of a Macaca fascicularis female of Mauritian origin using a whole-genome shotgun sequencing approach. We applied a template switch strategy which employs either the rhesus or the human genome to assemble sequence reads. The 6-fold sequence coverage of the draft genome sequence enabled discovery of about 2.1 million potential single-nucleotide polymorphisms based on occurrence of a dimorphic nucleotide at a given position in the genome sequence. Homology-based annotation allowed us to identify 17,387 orthologs of human protein-coding genes in the M. fascicularis draft genome and the predicted transcripts enabled the design of a M. fascicularis-specific gene expression microarray. Using liver samples from 36 individuals of different geographic origin, we identified 718 genes with highly variable expression in liver, whereas the majority of the transcriptome shows relatively stable and comparable expression. Knowledge of the M. fascicularis draft genome is an important contribution to both the use of this animal in disease models and the safety assessment of drugs and their metabolites. In particular, this information allows high-resolution genotyping and microarray-based gene expression profiling for animal stratification, thereby allowing the use of well-characterized animals for safety testing. Finally, the genome sequence presented here is a significant contribution to the global "3R" animal welfare initiative, which has the goal to reduce, refine and replace animal experiments. A 36-microarray study using total RNA recovered from liver samples of untreated Cynomolgus monkeys of good laboratory practice (GLP) drug safety studies. The monkeys were from the Philippines, a Chinese colony, and Mauritius. Each microarray measures the expression level of 16,896 genes using 20,047 probe sets with six 60-mer probes (PM) per probe set. Each probe set is represented once on the array. The Cynomolgus monkey gene expression results analyzed in this study are further described in Ebeling et al. (2011) (PMID 21862625).
2011-08-24 | E-GEOD-30184 | biostudies-arrayexpress
Project description:microRNA profiling from kidney cortex samples of Macaca fascicularis (Cynomolgus Monkey)
| PRJNA342671 | ENA
Project description:Effects of equol on gene expression in female cynomolgus monkey iliac arteries
| PRJNA158627 | ENA
Project description:Transcriptome profiling in the cynomolgus monkey hippocampus after acute and chronic methamphetamine administration
| PRJNA354893 | ENA
Project description:We studied time course of pathological remodeling occurring in the hearts of cynomolgus monkey, Macaca fascicularis against atrioventricular block condition. We used microarrays to detail the pathological changes in the hearts which had obtained sensitivity for detecting dl-sotalol-induced TdP by 7th month after the production of atrioventricular block.
2023-01-05 | GSE199943 | GEO
Project description:Blockade of thymic stromal lymphopoietin receptor (TSLPR) reduces allergic inflammation in a cynomolgus monkey model of asthma
| PRJNA176555 | ENA
Project description:The phase one of the cynomolgus monkey chromosome level genome assembly
| PRJEB38384 | ENA
Project description:Experimental animal models have played an indispensable role in the development of human induced pluripotent stem cell (iPSC) research. The, derivation of high-quality (so-called “true Naive state”) iPSCs of non-human primates enhances their application and safety for human regenerative medicine. Although several attempts have been made to convert human and non-human primate PSCs into a truly Naive state, it is unclear which evaluation methods can discriminate them as being truly Naive. Here we derived Naive-like Cynomolgus monkey (Cm) (Macaca fascicularis) embryonic stem cells (ESCs) and iPSCs, and evaluated their potential as a highly accurate indicator of the properties of Naive-like human ESC/iPS cells. Several characteristics of Naive-like Cm ESCs including colony morphology, teratoma-forming ability, leukaemia inhibitory factor dependency, and mitochondrial respiration were confirmed. Next, we generated Cm iPSCs and converted them to a Naive-like state. When these were subjected to in vitro differentiation, some exhibited heterogeneity and showed resistance to differentiation. The difficulty of achieving chimeric contribution to mouse or rabbit embryos suggested incomplete Naive conversion. Actually, transcriptomic comparison of PSCs with early Cm embryos elucidated the partial achievement of Naive conversion. This study offers valuable pointers for deriving true Naive PSCs while avoiding irrelevant or misleading evidence of Naive-like features.
2017-04-20 | GSE87576 | GEO
Project description:CGH analysis of monkey PG-haESCs (parthenogenic haploid embryonic stem cells)
| PRJNA188289 | ENA