Saccharomyces cerevisiae
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA348115 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Transcriptome analyses of dhh1 mutants in yeast
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Project description:Ribosome profiling of whi3 mutant yeast
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Project description:Ribosome profiling study of Dhh1p overexpression and the dhh1 knockout strain using monosome-protected footprints
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Project description:Determinants of ribosome density in yeast
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Project description:The impact of RNA structures in coding sequences (CDS) within mRNAs is poorly understood. Here
we identify a novel and highly conserved mechanism of translational control involving RNA structures within coding sequences and the DEAD-box helicase Dhh1. Using yeast genetics and
genome-wide ribosome profiling analyses we show that this mechanism, initially derived from studies
of the Brome Mosaic virus RNA genome, extends to yeast and human mRNAs highly enriched in
membrane and secreted proteins. All Dhh1-dependent mRNAs, viral and cellular, share key common
features. First, they contain long and highly structured CDSs, including a region located around 42 to
120 nucleotides after the translation initiation site, second, they are directly bound by Dhh1 with a
specific binding distribution and third, complementary experimental approaches suggest that they are
activated by Dhh1 at the translation initiation step. Our results show that ribosome translocation is not the only unwinding force of CDS and uncover a conserved layer of translational control that involve
RNA helicases and RNA folding within CDS providing novel opportunities for regulation of
membrane and secretome proteins.
2016-11-04 | GSE87892 | GEO
Project description:The impact of RNA structures in coding sequences (CDS) within mRNAs is poorly understood. Here we identify a novel and highly conserved mechanism of translational control involving RNA structures within coding sequences and the DEAD-box helicase Dhh1. Using yeast genetics and genome-wide ribosome profiling analyses we show that this mechanism, initially derived from studies of the Brome Mosaic virus RNA genome, extends to yeast and human mRNAs highly enriched in membrane and secreted proteins. All Dhh1-dependent mRNAs, viral and cellular, share key common features. First, they contain long and highly structured CDSs, including a region located around nucleotide 70 after the translation initiation site, second, they are directly bound by Dhh1 with a specific binding distribution and third, complementary experimental approaches suggest that they are activated by Dhh1 at the translation initiation step. Our results show that ribosome translocation is not the only unwinding force of CDS and uncover a novel layer of translational control that involves RNA helicases and RNA folding within CDS providing novel opportunities for regulation of membrane and secretome proteins.
2016-11-04 | GSE87888 | GEO
Project description:RNAseq and ribosome profiling of yeast cells grown under methionine restriction condition.
| PRJNA356775 | ENA
Project description:High-resolution view of the yeast meiotic program revealed by ribosome profiling
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Project description:The canonical role of eEF1A is to deliver the aminoacyl tRNA to the ribosome, we have used the yeast model system to investigate further roles for this protein. We used microarray to study the transcriptomic effects of elevated levels of eEF1A on yeast cells during log phase growth
2016-10-29 | GSE89286 | GEO
Project description:Degradation of most yeast mRNAs involves decapping by Dcp1/Dcp2. DEAD-box protein Dhh1 has been implicated as an activator of decapping, and as a translational repressor, but their functions in cells are incompletely understood. We have analyzed these questions by a combination of ribosome profiling, RNA-Seq, CAGE analysis of capped mRNAs.
2023-02-07 | GSE220578 | GEO