Project description:Methane is a well-known factor in green house effect and also could be an alternative energy, which produced via methanogenesis by methanogenic archaea. Methanohalophilus portucalensis FDF1T is a halophilic methylotrophic methanogen and has been used as a model to study osmoadaptation in fluctuated abiotic environmental conditions. Here we report a genome-wide phosphoproteomic analysis of M. portucalensis FDF1T to uncover the possible phosphorylation-mediated regulations in methanogenesis and osmoadaptation. According to the biological function analysis revealed 20.6% of total 149 phosphoproteins were involved in the regulation of the single carbon metabolism for methanogenesis and osmoadaptation. Together with genome sequencing and phosphoproteomic analysis, we provided the first evidence to indicate protein phosphorylation networks in regulating the pyrrolysine (Pyl) mediated translation of methylotrophic methyltransferase. Furthermore, the functional assays of glycine sarcosine N-methyltransferase revealed that the enzymatic activity was modulated by threonine phosphorylation. Collectively, our results not only provide new insight into phosphorylation-mediated regulations in single carbon energy metabolism but also clearly demonstrate that phosphorylation plays a critical role in osmolyte betaine synthesis through a phospho-switch mechanism.

2016-07-05 | PXD002024 | Pride

Transcriptional analysis of freshwater bacterium Polynucleobacter asymbioticus strain QLW-P1DMWA-1T

Project description:Polynucleobacter asymbioticus strain QLW-P1DMWA-1T represents a group of highly successful heterotrophic planktonic bacteria, dwelling in freshwater systems (lakes, ponds, and streams) across all climatic zones and across all continents. This includes habitats characterised by strongly fluctuating environmental conditions. So the experiments were designed to mimick winter and summer scenarios with additional impact of UV irradiation. Comparative transcriptomic studies were conducted to analyse gene-expression levels in contrasting experimental conditions. Overall, molecular candidates were revealed that may contribute in rapid acclimatisation of this strain in their immediate environment.

2017-10-31 | GSE98129 | GEO

Methanohalophilus halophilus strain:Z-7982 | isolate:Z-7982 | breed:Prokaryotic | cultivar:Methanohalophilus halophilus

Project description:Methanohalophilus halophilus strain:Z-7982 | isolate:Z-7982 | breed:Prokaryotic | cultivar:Methanohalophilus halophilus Genome sequencing

| PRJNA342410 | ENA

Citrobacter portucalensis strain:ECTV13 | isolate:Marine turtle

Project description:Citrobacter portucalensis strain:ECTV13 | isolate:Marine turtle Genome sequencing and assembly

| PRJNA489393 | ENA

Citrobacter portucalensis strain:TV06 | isolate:Marine turtle

Project description:Citrobacter portucalensis strain:TV06 | isolate:Marine turtle Genome sequencing and assembly

| PRJNA489514 | ENA

Methanohalophilus

Project description:Methanohalophilus sp. overview

| PRJNA530307 | ENA