Project description:To determine the genes directly and indirectly under the control of the Grainy-head homolog (GHH) transcription factor in Neurospora crassa Three different sample types (Aerial Hyphae & Conidia; Mycelia; or Whole Colonies) of both wild-type (FGSC #2489) and grainy-head homolog (FGSC #13563) strains of Neurospora crassa were subjected to transcriptome analyses to determine the genes differentially expressed in the ghh background compared to wild type.

2012-03-01 | E-GEOD-35017 | biostudies-arrayexpress

Neurospora crassa strain:FGSC 2489 dNCU00890

Project description:Neurospora crassa FGSC 2489 Gene Expression Profiling - dNCU00890 2mM mannobiose-1 (445)

| PRJNA349718 | ENA

Neurospora crassa strain:FGSC 2489 dNCU00890

Project description:Neurospora crassa FGSC 2489 Gene Expression Profiling - dNCU00890 2mM mannobiose-2 (446)

| PRJNA349719 | ENA

RNA-sequencing of WT and ΔMSN1 strains of N. crassa over a circadian time course

Project description:We performed high throughput sequencing of the transcriptome of N. crassa to quantify ultradian (<24h period) rhythms in the wild-type (FGSC#2489) and NCU02671 (msn-1) knockout (FGSC#11345) strains.

2018-08-01 | GSE113845 | GEO

Neurospora crassa early sexual development with oligod(T) priming

Project description:Multi-targeting priming (MTP) for genome-wide gene expression assays provides selective targeting of multiple sequences and counter-selection against undesirable sequences. We demonstrated superior performance of two MTPs compared to oligo-dT microarray profling and RNA tag sequencing the response of Saccharomyces cerevisiae to nitrogen deficiency and profiling Neurospora crassa early sexual development. Priming with MTPs in addition to oligo-dT resulted in higher sensitivity, a greater number of well-measured genes, more genes significantly differentially expressed, and a greater power to detect meager differences. Neurospora crassa mat A FGSC#2489 2 developmental stages and oligo(dT) primers.

2010-07-17 | E-GEOD-22972 | biostudies-arrayexpress

Mating-type specific gene expression in the filamentous ascomycete Neurospora tetrasperma

Project description:Neurospora tetrasperma is a pseudohomothallic filamentous ascomycete with a large (~ 7 Mbp) region of suppressed recombination surrounding its mating-type (mat) locus. The suppressed recombination has lead to sequence divergence between the two mating-type chromosomes of wild-type heterokaryotic strains, while the remaining genome is largely homoallelic. In this study, we use microarray technology to manifest expression divergence linked to mating type in N. tetrasperma. N. tetrasperma and N. crassa, were grown on agar regimes inducing sexual growth (Synthetic Crossing medium) and vegetative growth (Vogel's Medium), respectively. [SC]: Neurospora tetrasperma mat-A FGSC#1270; mat-a FGSC#1271; Mat-A FGSC#9033; mat-a FGSC#9034; N. crassa mat-A FGSC#2489 and mat-a FGSC 4200: Synthetic Crossing medium was used as a nutrient regime before sampling and processing [Veg]: Neurospora tetrasperma mat-A FGSC#1270; mat-a FGSC#1271; Mat-A FGSC#9033; mat-a FGSC#9034; N. crassa mat-A FGSC#2489 and mat-a FGSC 4200: Vogel's Medium (Vegetative Medium) was used as a nutrient regime before sampling and processing

2011-01-26 | E-GEOD-26798 | biostudies-arrayexpress

Identification of the transcriptional profile of the hyphal tip of Neurospora crassa

Project description:A colony of fungus is comprised of long, branching filamentous cells called hyphae. Genetic mechanisms underlying the development of hyphae are poorly understood. We sectioned hyphae of a model fungus, Neurospora crassa (pink bread mold), into six parts depending on the age of the cells; 1 hour, 3 hour, 9 hour, 15 hour, 21 hour and 27 hour old, respectively. This data submission reflects an RNAseq analysis of mRNA extracted from the 1 hour time-point. 1 mycelial mRNA profile of 1 hour growth (hyphal tip) from Neurospora crassa strain FGSC 2489, Data was generated using Illumina GAIIx.

2013-08-28 | E-GEOD-50205 | biostudies-arrayexpress

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