Genomics

Dataset Information

118

Cell fixation and preservation for droplet-based single cell transcriptomics


ABSTRACT: Cell fixation and preservation for droplet-based single cell transcriptomics

PROVIDER: PRJNA350512 | ENA |

REPOSITORIES: ENA

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BACKGROUND:Recent developments in droplet-based microfluidics allow the transcriptional profiling of thousands of individual cells in a quantitative, highly parallel and cost-effective way. A critical, often limiting step is the preparation of cells in an unperturbed state, not altered by stress or ageing. Other challenges are rare cells that need to be collected over several days or samples prepared at different times or locations. METHODS:Here, we used chemical fixation to address these proble  ...[more]

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