Project description:Purpose: The goal of this study is to compare differences between Penicillium roqueforti conidia from various conditions shown to affect heat-resistance to identify genes possibly related to heat-resistance using RNA-seq. Methods: mRNA from Penicillium roqueforti conidia produced at different temperatures (15C, 25C or 30C) and different cultivation times (3, 5, 7 or 10 days) was extracted, in triplicate, and sequenced with Illumina NextSeq500. The sequence reads that passed quality filters were analyzed using Hisat2 followed by Cufflinks. Results: we mapped about 19 million sequence reads per sample to the Penicillium roqueforti genome (build LCP96 04111) and identified 9723 transcripts in the conidia. Data analysis revealed a subset of 33 genes showing increased expression in culture conditions that increase heat-resistance. Conclusions: Our study generated insight into multiple genes that are likely involved in the heat-resistance of Penicillium roqueforti.
Project description:Modulation of gut microbiota through probiotic supplementation is an interesting strategy to prevent obesity We use microarrays to study the global genome expression of C. elegans fed with the probiotic strain Bifidobacterium animalis sbsp. lactis CECT 8145