Project description:Brown algae (Phaeophyceae) are complex photosynthetic organisms with a very different evolutionary history to green plants, to which they are only distantly related. These seaweeds are the dominant species in rocky coastal ecosystems and they exhibit many interesting adaptations to these, often harsh, environments. The brown algae are also important because they are one of only a very small number of eukaryotic lineages that have evolved complex multicellularity. This work used whole genome tiling array approach to generate a comprehensive transcriptome map of the filamentous seaweed Ectocarpus siliculosus (Dillwyn) Lyngbye, a model organism for the brown algae. Keywords: high-resolution tiling array, brown algae, ectocarpus
Project description:Transcriptomic study for the response of Ectocarpus siliculosus to mild copper stress (250 µg L-1 CuCl2) after 4h and 8h of exposure
Project description:Reference reference hybridization with Ectocarpus siliculosus (strain Es 32) genomic DNA; Quality control for submission E-TABM-766
Project description:Brown algae (Phaeophyceae) are complex photosynthetic organisms with a very different evolutionary history to green plants, to which they are only distantly related. These seaweeds are the dominant species in rocky coastal ecosystems and they exhibit many interesting adaptations to these, often harsh, environments. The brown algae are also important because they are one of only a very small number of eukaryotic lineages that have evolved complex multicellularity. This work used whole genome tiling array approach to generate a comprehensive transcriptome map of the filamentous seaweed Ectocarpus siliculosus (Dillwyn) Lyngbye, a model organism for the brown algae. Keywords: high-resolution tiling array, brown algae, ectocarpus The slides were hybridised with two, labelled samples: 1) a mixture of labelled cDNA corresponding to RNA samples from mature sporophytes and gametophytes and from immature sporophytes stressed either in high salt medium or by addition of hydrogen peroxide and 2) genomic DNA as a control.
Project description:Comparative Genome Hybridization of Ectocarpus siliculosus strains 371 (freshwater ecotype), 524 (copper-tolerant strain), and 568 (female strain), as well as E. fasciculatus strain 395 against the sequenced genome strain (32).
Project description:The aim of the experiment is to identify Ectocarpus siliculosus (strain Ec32) genes which are up- or down-regulated by auxin. RNAs were extracted from sporophytes treated with auxin NAA 5.10-6M for 30min or 3h, and labelled either with Cy3 or Cy5. Biological triplicates were performed.
