Oryza sativa Japonica Group
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA354794 | ENA |
REPOSITORIES: ENA
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Project description:Background: Partial pollen and embryo sac sterilities are the two main reasons for low fertility in autotetraploid rice. Our previous study revealed that small RNAs changes may associate with pollen fertility in autotetraploid rice. However, knowledge on comparative analysis between the development of pollen and embryo sac by small RNAs in autotetraploid rice is still unknown. In the present study, WE-CLSM (whole-mount eosin B-staining confocal laser scanning microscopy) and high-throughput sequencing technology was employed to examine the cytological variations and to analyze small RNAs changes during pollen and embryo sac development in autotetraploid rice compared with its diploid counterpart. Results: A total of 321 and 368 differentially expressed miRNAs (DEM) were detected during development of pollen and embryo sac in autotetraploid rice, respectively. Gene Ontology enrichment analysis on the targets of miRNAs-enriched during the development of pollen and embryo sac in autotetraploid rice revealed 30 prominent functional gene classes, such as cell differentiation and signal transduction during embryo sac development. However, only 7 prominent functional gene classes, such as flower development and transcription factor activity, were detected during pollen development. The expression levels of 39 DEM, which revealed interaction with meiosis-related genes, showed opposite expression levels in pollen and embryo sac development. Of these DEM, osa-miR1436_L+3_1ss5CT and osa-miR167h-3p were associated with the female meiosis, while osa-miR159a.1 and osa-MIR159a-p5 were related with the male meiosis. 21nt-phasiRNAs were detected both during pollen and embryo sac development, while 24nt-phasiRNAs were found only in pollen development, which displayed down-regulation in autotetraploid compared to diploid rice and their spatial-temporal expression patterns were similar to osa-miR2275d. 24nt TEs-siRNAs were found to be up-regulated in embryo sac but down-regulated in pollen development. Conclusion: The above results not only provide the small RNAs changes during four landmark stages of pollen and embryo sac development in autotetraploid rice but also have identified specifically expressed miRNAs, especially meiosis-related miRNAs, pollen-24nt-phasiRNAs and TEs-siRNAs in autotetraploid rice. Together, these findings provide a foundation for understanding the effect of polyploidy on small RNAs expression patterns during pollen and embryo sac development that may lead to different abnormalities in autotetraploid rice.
2017-03-09 | GSE90479 | GEO
Project description:MicroRNAs (miRNAs) play key roles in plant reproduction. However, knowledge on microRNAome analysis in autotetraploid rice is rather limited. Here, high-throughput sequencing technology was employed to analyze miRNAomes during pollen development in autotetraploid rice. A total of 172 differentially expressed miRNAs (DEM) were detected in autotetraploid rice compared to its diploid counterpart, and 57 miRNAs were specifically expressed in autotetraploid rice. Of the 172 DEM, 115 and 61 miRNAs were found to be up-regulated and down-regulated, respectively. Gene Ontology analysis on the targets of up-regulated DEM showed that they were enriched in transport and membrane in pre-meiotic interphase, reproduction in meiosis, and nucleotide binding in single microspore stage. osa-miR5788 and osa-miR1432-5p_R+1 were up-regulated in meiosis and their targets revealed interaction with the meiosis-related genes, suggesting that they may involve in the genes regulation associated with the chromosome behavior. Abundant 24-nt siRNAs associated with transposable elements were found in autotetraploid rice during pollen development; however, they significantly declined in diploid rice, suggesting that 24-nt siRNAs may play a role in pollen development. These findings provide a foundation for understanding the effect of polyploidy on small RNA expression patterns during pollen development that lead to low pollen fertility in autotetraploid rice.
2016-11-04 | GSE79344 | GEO
Project description:In this study, we identified the genes specifically/preferentially expressed in maize embryo sac and genes differentially expressed before and after pollination in maize embryo sac via RNA-seq. In total, more than 30% of them are unknown functions and more than 80% of which are first reported in maize embryo sac. Examination of 3 different tissues of maize to provide a comprehensive and much more important data for further analysis of embryo sac-pollen tube interactions.
2015-04-25 | E-GEOD-57075 | biostudies-arrayexpress
Project description:We perform a quantitative RNA-seq analysis of embryo sacs, comparator ovules with the embryo sacs removed, mature pollen, and seedlings to assist the identification of gametophyte functions in maize. Expression levels were determined for annotated genes in both gametophytes, and novel transcripts were identified from de novo assembly of RNA-seq reads. RNA-seq was performed on four tissue types: nine-day old, above-ground seedling (S); mature pollen (MP); embryo-sac-enriched samples with some remaining nucellar cells (ES); and ovules with embryo sacs removed (Ov).
2011-07-09 | E-MTAB-4128 | biostudies-arrayexpress
Project description:Comparative small RNA analysis of pollen development in autotetraploid and diploid rice
| PRJNA315549 | ENA
Project description:Mutation of the ASHH2 gene has pleiotropic developmental effects. We have focused on the role of ASHH2 in plant reproduction.The aim of the study was to identify changes in gene expression in inflorescences of the ashh2-1/sdg8-1 mutant (SALK_065480, insertion in the gene At1g77300, SDG8/EFS/ASHH2) compared to wt Arabidopsis thaliana (ecotype Colombia) inflorescences. On the female side, close to 80% of mature ovules lack embryo sac. On the male side, anthers frequently develop without pollen sacs or with specific defects in the tapetum layer, resulting in reduction in the number of functional pollen per anther by up to ~90%. ashh2 mutations also result in homeotic changes in floral organ identity. Transcriptional profiling identified more than 300 up-regulated and 600 down-regulated genes in ashh2 mutant inflorescences, whereof the latter included genes involved in determination of floral organ identity, embryo sac and anther/pollen development. This was confirmed by real-time PCR. In the chromatin of such genes (AP1, AtDMC1 and MYB99) we observed a reduction of H3K36 trimethylation (me3), but not H3K4me3 or H3K36me2.
2009-10-10 | GSE18325 | GEO
Project description:Fertile pollen is critical for the survival, fitness and dispersal of flowering plants, and directly contributes to crop productivity. Extensive mutational screening studies have been carried out to dissect the genetic regulatory network determining pollen fertility, but we still lack fundamental knowledge about whether and how pollen fertility is controlled in natural populations. We used a genome-wide association study (GWAS) to show that ZmGEN1A and ZmMSH7, two DNA repair-related genes, confer natural variation in maize pollen fertility. Mutants defective in both genes exhibited abnormalities in meiotic or post-meiotic DNA repair, leading to reduced pollen fertility. More importantly, ZmMSH7 underwent selection during maize domestication, and its disruption resulted in a substantial increase in grain yield and protein content for both inbred and hybrid. Overall, our study describes the first systematic examination of natural genetic effects on pollen fertility in plants, providing valuable genetic resources for optimizing male fertility. Moreover, ZmMSH7 may be a potential candidate for simultaneous improvement of grain yield and quality.
2024-02-09 | GSE224891 | GEO
Project description:In this study, we identified the genes specifically/preferentially expressed in maize embryo sac and genes differentially expressed before and after pollination in maize embryo sac via RNA-seq. In total, more than 30% of them are unknown functions and more than 80% of which are first reported in maize embryo sac.
2015-04-25 | GSE57075 | GEO
Project description:Using the Illumina HiSeq 2000 platform, 39,598; 32,403and 42,208 genes were identified in flower buds of B. carinata cv.W29, B. napus cv. Zhongshuang 11 and their hybrids, respectively. The differentially expressed genes (DEGs) were significantly enriched in pollen wall assembly, pollen exine formation, pollen development, pollen tube growth, pollination, gene transcription, macromolecule methylation and translation, which might be associated with impaired fertility in the F1 hybrid. These results will shed light on the mechanisms underlying the low fertility of the interspecific hybrids and expand our knowledge of interspecific hybridization.
2016-11-05 | GSE63088 | GEO
Project description:Newly synthetic autotetraploid rice generally shows lower pollen fertility and seed setting rate in comparison to diploid rice, and this can hinder its own domestication and breeding. In this study, cytological analysis was used to demonstrate the imbalanced separation of homologous chromosomes occurred at meiosis I during the pollen mother cell development, and the early degeneration of tapetum cells in autotetraploid rice. We identified a total of 941 differentially expressed proteins (DEPs) in anthers, including 489 up-regulated and 452 down-regulated proteins. KOG analysis showed that the identified DEPs were related to post-translational modifications, such as protein ubiquitination. These modifications are related to chromatin remodeling and homologous recombination abnormalities during meiosis. The key proteins related to the pentose phosphate pathway (BGIOSGA016558, BGIOSGA022166, BGIOSGA028743) are significantly down-regulated, which can affect the failure of autotetraploid rice to provide the energy needed for cell development after polyploidization, and ultimately lead to the early degradation of the tapetum. Proteins (BGIOSGA017346and BGIOSGA027368) related to the degradation of glutenin are up-regulated, indicating that the decomposition of glutenin can lead to the early degradation of tapetum. These cytological and molecular evidences will help to promote our understanding of the differences in anther development between diploid and autotetraploid rice during meiosis.
2022-04-12 | PXD033183 | iProX