Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data of neuroblastoma tissues, spheres and NGP cell line overexpressing CFC1

ABSTRACT: Expression data of neuroblastoma tissues, spheres and NGP cell line overexpressing CFC1

PROVIDER: PRJNA355908 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Expression data of neuroblastoma tissues, spheres and NGP cell line overexpressing CFC1

Project description:This SuperSeries is composed of the SubSeries listed below.

2017-12-01 | GSE90791 | GEO

Expression data of CFC1 overexpressed neuroblastoma cell line, NGP

Project description:We performed a microarray analysis on CFC1-expressing NGP cells. Expression data in CFC1- and mock-NGP cells were generated and compared. Differentially expressed genes (DEGs) were functionally annotated by a pathway analysis and Gene Set Enrichment Analysis (GSEA). Both analyses showed that genes involved in “differentiation”, “oncogene-induced senescence”, “stem cell proliferation” and “Activin signaling” pathways were significantly enriched in DEGs. “TGFbeta pathway” and “Nodal pathway” were also enriched in the GeneSpring GX pathway analysis, but were not significant in the GSEA analysis. This result suggests that the pathways involved in cell stemness and Activin signaling were enhanced by the expression of CFC1 in NB cells accompanied by transcriptional changes in their related gene members.

2017-12-01 | GSE90790 | GEO

Identification of SOX11 binding sites through CUT&RUN sequencing in the neuroblastoma cell lines IMR-32, CLB-GA, NGP and SH-EP

Project description:CUT&RUN sequencing to define binding sites for SOX11 and IgG in the neuroblastoma cell lines IMR-32, CLB-GA and NGP as well as neuroblastoma cell line SH-EP after SOX11 overexpression for 48h. A SOX11 inducible overexpression system was generated using a Tet-on system.

2022-12-13 | E-MTAB-11905 | biostudies-arrayexpress

RNA-seq of human CLB-GA and NGP neuroblastoma cell lines with SOX11 knockdown as compared to control

Project description:RNA-seq upon SOX11 knockdown in the neuroblastoma cell lines CLB-GA and NGP. Cells were nucleofected with a pool of four different siRNAs targeting SOX11 and a non-targeting control (NTC). Analysis was performed 2 days upon SOX11 knockdown, including four biological replicates per condition.

2022-12-13 | E-MTAB-11883 | biostudies-arrayexpress

ERK inhibitor, ulixertinib induces transcriptomic changes in NGP cells

Project description:To investigate the effect of ERK inhibitor, ulixertinib, on NB cell proliferation and delineate the mechanisms of ulixertinib-mediated ERK pathway inhibition in NB. We treated human neuroblastoma cell line, NGP with ulixertinib and performed gene expression profiling analysis using data obtained from RNA-seq.

2023-03-01 | GSE213153 | GEO

Global gene expression analysis in NGP CRISPR control cells and NGP ALK(LF655del) cells.

Project description:We performed gene expression profiling by Affymetrix primeview array in both NGP CRISPR control cells and NGP ALK(LF655del) cells.

2021-08-11 | GSE140025 | GEO

Reversible LSD1 Inhibition with HCI-2509 induces the p53 gene expression signature in high-risk neuroblastoma cells

Project description:Lysine-Specific Demethylase 1 (LSD1) over-expression correlates with poorly differentiated neuroblastoma and predicts poor outcome despite multimodal therapy. We have studied the efficacy of reversible and specific LSD1 inhibition with HCI-2509 in neuroblastoma cell lines and particularly the effect of HCI-2509 on the transcriptomic profile in MYCN amplified NGP cells. Cell survival assays show that HCI-2509 is cytotoxic to poorly differentiated neuroblastoma cell lines in low micromole or lower doses. Transcriptional profiling of NGP cells treated with HCI-2509 shows a significant effect on p53, cell cycle, MYCN and hypoxia pathway gene sets. HCI-2509 results in increased histone methyl marks and p53 levels along with cell cycle arrest in the G2/M phase and inhibition of colony formation of NGP cells. Our findings indicate that LSD1 inhibition with HCI-2509 has a multi-target effect in MYCN amplified high-risk neuroblastoma cells.

2019-01-01 | GSE104526 | GEO

The proteome of neuroglobin-overexpressing SH-SY5Y neuroblastoma cells

Project description:Neuroglobin (NGB) is an oxygen-binding globin expressed in vertebrates mainly in the central and peripheral nervous system, but also in extra-neuronal cells. NGB is involved in oxygen transport, storage, and homeostasis. The role hypothesized for this globin is in fact related to neuroprotection against oxidative stress. Also, NGB is functionally and physically associated to mitochondria, possibly playing a role in ATP production and regulation. NGB actually exerts its function upon up-regulation. Thus, NGB-overexpressing SH-SY5Y neuroblastoma cells (NGB-FLAG) were analyzed by shotgun label-free quantitative proteomics in comparison with control cells (CTRL).

2021-12-20 | PXD029012 | Pride

Genome-wide binding of MYCN protein in MYCN-amplified neuroblastoma cell lines

Project description:The MYCN locus is amplified in about half of high-risk neuroblastoma tumors. To identify genomic loci occupied by MYCN protein in the MYCN-amplified neuroblastoma cell lines NGP, Kelly and NB-1643, we performed chromatin immunoprecipitation coupled with Next-Generation Sequencing (ChIP-seq) using an anti-MYCN antibody.

2017-08-02 | GSE94782 | GEO

SMARTer single cell total RNA sequencing [NGP]

Project description:single cell polyA+ and total RNA-seq data was generated for NGP, SK-N-BE-2C and SHSY5Y-MYCN-TR cells

2019-01-01 | GSE119936 | GEO