Project description:This is an ordinary differential equation mathematical model investigating the early responses of human monocyte-derived dendritic cells to infection by two H1N1 influenza A viruses of different clinical outcomes: pandemic A/California/4/2009 and seasonal A/New Caledonia/20/1999.
Project description:Transcriptome analysis of three different populations of human monocyte-derived myeloid cells: Autologous Tolerogenic Dendritic Cells (ATDCs), Monocyte-derived Dendritic Cells (MoDCs) and Monocyte-derived Macrophages (MoMacro)
Project description:To examine the miRNA changes that occur during macrophage and dendritic cell differentiation we used the miRCURY LNATM microRNA Array (7th Gen) to profile monocyte-derived macrophages and monocyte-derived dendritic cells. Of the 2046 probes present on the assay, we detected expression of ~400 (20%) miRNAs across all samples.
Project description:We have carried out global gene expression analysis to clarify the interrelationship between 1,25-dihydroxyvitamin D3 and differentiation-driven gene expression patterns in developing human monocyte-derived dendritic cells. Monocytes were treated with 10 nM 1,25-dihydroxyvitamin D3 or vehicle 14 hours after plating for 12 hours or 5 days. Monocytes, differentiating dendritic cells (+/-1,25-dihydroxyvitamin D3 for 12 hours) and immature dendritic cells (+/-1,25-dihydroxyvitamin D3 for 5 days) were harvested. This design allows one to identify genes regulated by differentiation and/or 1,25-dihydroxyvitamin D3 in human monocyte-derived dendritic cells.
Project description:Transcriptional profiling of human monocyte-derived dendritic cells, comparing cells transfected for 24h with Pre-miR-650 or nonsense control.
