Project description:Investigation of gene expression level changes in snapdragon petals and sepals during flower development The flower developmental stages analyzed in this study are representative of distinct developmental events: (i) preanthesis, (ii) anthesis, (iii) maturation and (iv) presenescence and are further described in the accompanying article. A 24 chip study using total RNA recovered from samples of petal and sepal tissue of Antirrhinum majus cv. Maryland True Pink harvested at four different stages of flower development, namely (i) preanthesis (three days before flower opening=d-3), (ii) anthesis (day of flower opening=d1), (iii) maturation (four days after flower opening=d4) and (iv) presenescence (seven days after flower opening=d7). Three separate samples were extracted per tissue and developmental stages. Each chip measures the expression level of 11,959 ESTs from Antirrhinum majus cv. Maryland True Pink with up to six 60-mer probes per target.
Project description:Our aim was to study quantitative and qualitative small RNA differences between sulf and SULF+ plants. We used sulf-660 and its revertant, derived by transposon excision from the sulf-660 allele, to minimise background variation and highlight key differences associated with the SULF locus.
Project description:Investigation of gene expression level changes as a result of crowding growth conditions. A five-chip study using total RNA recovered from samples of inflorescence meristems of Antirrhinum majus cv.165E, grown as single plant per pot (control) and 10 plants per pot (crowded conditions). Each chip measures the expression level of 11,959 ESTs with up to six 60-mer probes per target.