Enterococcus faecalis
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA357821 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:GenomeTrakr Project: NSF International | Applied Research Center
| PRJNA357820 | ENA
Project description:GenomeTrakr Project: NSF International | Applied Research Center
| PRJNA357818 | ENA
Project description:GenomeTrakr Project: NSF International | Applied Research Center
| PRJNA357822 | ENA
Project description:GenomeTrakr Project: NSF International | Applied Research Center
| PRJNA514294 | ENA
Project description:GenomeTrakr Project: NSF International | Applied Research Center
| PRJNA357819 | ENA
Project description:GenomeTrakr wastewater project: US FDA, Center for Food Safety and Applied Nutrition
| PRJNA757447 | ENA
Project description:GenomeTrakr wastewater project: US FDA, Center for Food Safety and Applied Nutrition
| PRJNA761415 | ENA
Project description:GenomeTrakr Project: Enterococcus sp., New York State Department of Health, Wadsworth Center
| PRJNA742919 | ENA
Project description:Sharing of research data has begun to gain traction in many areas of the sciences in the past few years because of changing expectations from the scientific community, funding agencies, and academic journals. National Science Foundation (NSF) requirements for a data management plan (DMP) went into effect in 2011, with the intent of facilitating the dissemination and sharing of research results. Many projects that were funded during 2011 and 2012 should now have implemented the elements of the data management plans required for their grant proposals. In this paper we define 'data sharing' and present a protocol for assessing whether data have been shared and how effective the sharing was. We then evaluate the data sharing practices of researchers funded by the NSF at Oregon State University in two ways: by attempting to discover project-level research data using the associated DMP as a starting point, and by examining data sharing associated with journal articles that acknowledge NSF support. Sharing at both the project level and the journal article level was not carried out in the majority of cases, and when sharing was accomplished, the shared data were often of questionable usability due to access, documentation, and formatting issues. We close the article by offering recommendations for how data producers, journal publishers, data repositories, and funding agencies can facilitate the process of sharing data in a meaningful way.
| S-EPMC4757565 | BioStudies
Project description:Enterococcus faecalis are hospital-associated opportunistic pathogens and also causative agents of post-operative endophthalmitis. Patients with enterococcal endophthalmitis often have poor visual outcomes, despite appropriate antibiotic therapy. Here we investigated the genomic and phenotypic characteristics of E. faecalis isolates collected from 13 patients treated at the University of Pittsburgh Medical Center Eye Center over 19 years. Comparative genomic analysis indicated that patients were infected with E. faecalis belonging to diverse multi-locus sequence types (STs) and resembled E. faecalis sampled from clinical, commensal, and environmental sources. We identified known E. faecalis virulence factors and antibiotic resistance genes in each genome, including genes conferring resistance to aminoglycosides, erythromycin, and tetracyclines. We assessed all isolates for their cytolysin production, biofilm formation, and antibiotic susceptibility, and observed phenotypic differences between isolates. Fluoroquinolone and cephalosporin susceptibilities were particularly variable between isolates, as were biofilm formation and cytolysin production. In addition, we found evidence of E. faecalis adaptation during recurrent endophthalmitis by identifying genetic variants that arose in sequential isolates sampled over eight months from the same patient. We identified a mutation in the DNA mismatch repair gene mutS that was associated with an increased rate of spontaneous mutation in the final isolate from the patient. Overall this study documents the genomic and phenotypic variability among E. faecalis causing endophthalmitis, as well as possible adaptive mechanisms underlying bacterial persistence during recurrent ocular infection.
| S-EPMC8046195 | BioStudies