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Paenibacillus larvae subsp. larvae strain:LMG 16252 | isolate:ERICIII

ABSTRACT: Paenibacillus larvae subsp. larvae strain:LMG 16252 | isolate:ERICIII Genome sequencing and assembly

PROVIDER: PRJNA358155 | ENA |

REPOSITORIES: ENA

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Project description:Paenibacillus larvae subsp. larvae B-3650 genome sequencing project

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The overall response of Paenibacillus larvae to bodily fluids of honeybee larvae (Apis mellifera).

Project description:Paenibacillus larvae, the causal agent of American Foulbrood disease (AFB), affects honeybee health worldwide. The present study investigates the transcriptional response of this Gram-positive, endospore-forming bacterium to bodily fluids from honeybee larvae. Four different conditions were evaluated with a loop design: sampling of in vitro grown P. larvae cultures one or four hours after addition of larval fluids or BHIT-broth (C1, T1, C4, T4).

2014-12-22 | GSE37481 | GEO

Paenibacillus larvae

Project description:Paenibacillus larvae overview

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Paenibacillus larvae exoproteins of ERIC I-IV genotypes

Project description:The following strains of Paenibacillus larvae classified as ERIC I-IV genotypes (pl1-pl4) were used in the study: ERIC I - DSM-7030; ERIC II - DSM-25430; ERIC III - LMG-16252; and ERIC IV - DSM-3615. The 7 biological replicates (a-g) of the exoprotein fractions prepared from each ERIC genotype were used in the nanoLC-MS/MS analysis. All 28 samples were consecutively analyzed using an OrbitrapTM FusionTM Tribrid q-OT-IT mass spectrometer. To improve the protein identification and easy exclude contaminants from the medium, we considered the proteins that could originate from the yeast extract and Mueller-Hinton broth (contains beef fusion and casein peptone-acidic hydrolysate) in analyzing the MS data. Thus, we included the sequences related to Saccharomyces cerevisiae and Bos taurus, respectively, to the list of contaminants in MaxQuant. The contaminants.fasta file is provided. Futhermore, we provide the database (uniprot-paenibacillus+larvae.fasta) used for the identification of P. larvae proteins created of different strains of P. larvae. The sequences were downloaded of UniProt on 12.03.2019. Finally, the compressed (zipped) folder "combined" is provided. The data were analyzed in MaxQuant v1.6.3.4.

2019-03-27 | MSV000083636 | MassIVE

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