Project description:Ethylene, as a signaling hormone molecule, is proved to have essential role in the process of root development. In the present study, cucumber (Cucumis sativus L.) seedlings were employed to estimate differentially expressed proteins (DEPs) during the adventitious rooting using iTRAQ technique and proteomics analysis. Out of the 5014 DEPs, 115 DEPs were considered as identified proteins, and among them, 24 DEPs are interesting proteins abundance.
Project description:To elucidate the functional role of the small signaling peptide RALF34 in the parental root meristem and its role in lateral root initiation, we studied changes in Cucumis sativus proteome during CsRALF34 gene overexpression. Composite plants with transgenic root systems carrying the construct for CsRALF34 overexpression (p35S::CsRALF34-TermAct) and control roots carrying p35S-GUS-TermAct were obtained and their proteome profiles were compared.