Project description:Castration-resistant prostate cancer (CRPC) that arise after the failure of androgen deprivation therapy is a leading cause of deaths in prostate cancer patients. HOXD-AS1 is reported to play a role in bladder cancer and neuroblastoma. However, its function and underlying mechanism in CRPC remains unknown. The goal of this study is to identify the target genes of HOXD-AS1 in prostate cancer. Our results inditcate that the genes regulated by HOXD-S1 involved in a variety of biological functions, such as proliferation and and Androgen Receptor signaling.
Project description:Prostate cancer is the most common cancer in men and AR downstream signalings promote prostate cancer cell proliferation. We identified a novel androgen-regulated long non-coding (lnc) RNA, SOCS2-AS1. In order to investigate the SOCS2-AS1 function in prostate cancer cells, we performed gene expression in AR-positive prostate cancer cell lines (LNCaP and LTAD) after siSOCS2-AS1 or siSOCS2 treatment. We also treated cells with vehicle or androgen to analyzed the effects of siSOCS2-AS1 on AR function. Observation of androgen dependent gene expression changes after treatmet with siSOCS2-AS1 with microarray.
Project description:Prostate cancer is the most common cancer in men and AR downstream signalings promote prostate cancer cell proliferation. We identified POTEF-AS1 is an androgen-regulated non-coding RNA gene. In order to investigate the POTEF-AS1 function in prostate cancer cells, we performed gene expression in AR-positive prostate cancer cell lines after siPOTEF-AS1 treatment. We also treated cells with vehicle or androgen to analyzed the effects of POTEF-AS1 on AR function.
