Project description:The draft genome of L. sativa (lettuce) cv. Tizian was sequenced in two Illumina sequencing runs, mate pair and shotgun. This entry contains the RAW sequencing data.

2018-02-09 | E-MTAB-6347 | biostudies-arrayexpress

Lentinus tigrinus strain:CONT

Project description:Lentinus tigrinus sp. CONT Standard Draft Genome

| PRJNA200609 | ENA

Epicoccum nigrum strain:cont 1108929

Project description:Epicoccum nigrum cont 1108929 Standard Draft genome sequencing

| PRJNA572470 | ENA

Phaeomoniellales sp. NC0930 Standard Draft

Project description:Phaeomoniellales sp. NC0930 genome sequencing

| PRJNA346873 | ENA

Penicillium sp. NC0857 Standard Draft

Project description:Penicillium sp. NC0857 genome sequencing

| PRJNA346348 | ENA

A synthetic control genome provides a universal molecular standard to measure DNA, mRNA and protein expression

Project description:The phi X 174 bacteriophage was first sequenced in 1977, and has since become the most widely used standard in molecular biology and next-generation sequencing. However, with the advent of affordable DNA synthesis and de novo gene design, we considered whether we could engineer a synthetic genome, termed SynX, specifically tailored for use as a universal molecular standard. The SynX genome encodes 21 synthetic genes that can be in vitro transcribed to generate matched mRNA controls, and in vitro translated to generate matched protein controls. This enables the use of SynX as a matched control to compare across genomic, transcriptomic and proteomic experiments. The synthetic genes provide qualitative controls that measure sequencing accuracy across k-mers, GC-rich and repeat sequences, as well as act as quantitative controls that measure sensitivity and quantitative accuracy. We show how the SynX genome can measure DNA sequencing, evaluate gene expression in RNA sequencing experiments, or quantify proteins in mass spectrometry. Unlike previous spike-in controls, the SynX DNA, RNA and protein controls can be independently and sustainably prepared by recipient laboratories using common molecular biology techniques, and widely shared as a universal molecular standard.

2024-03-22 | PXD035035 | Pride

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