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Escherichia coli strain:BW25113 JBx_072908

ABSTRACT: Escherichia coli BW25113 JBx_072908 resequencing genome sequencing

PROVIDER: PRJNA370058 | ENA |

REPOSITORIES: ENA

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E. coli BW25113 frdC vs. E. coli BW25113 HW2

Project description:Glycerol is an attractive feedstock for biofuels since it accumulates as a byproduct during biodiesel operations; hence, it is interesting to consider converting glycerol to hydrogen using the formate hydrogen lyase system of Escherichia coli which converts pyruvate to hydrogen. Starting with Escherichia coli BW25113 frdC that lacks fumarate reductase to eliminate the negative effect of accumulated hydrogen on glycerol fermentation and by using both adaptive evolution and chemical mutagenesis combined with a selection method based on increased growth on glycerol, we obtained an improved strain, HW2, that produces 20-fold more hydrogen in glycerol medium (0.68 mmol/L/h) compared to that of frdC mutant. HW2 also grows 5-fold faster (0.25 1/h) than BW25113 frdC on glycerol, so it achieves a reasonable growth rate. Corroborating the increase in hydrogen production, glycerol dehydrogenase activity in HW2 increased 4-fold compared to BW25113 frdC. In addition, a whole-transcriptome study revealed that several pathways that would decrease hydrogen yields were repressed in HW2 (fbp, focA, and gatYZ) while a beneficial pathway, eno which encodes enolase was induced.

2010-10-31 | GSE18972 | GEO

Escherichia coli strain:BW25113 JME44

Project description:Escherichia coli BW25113 JME44 resequencing genome sequencing

| PRJNA370162 | ENA

Escherichia coli strain:BW25113 JME45

Project description:Escherichia coli BW25113 JME45 resequencing genome sequencing

| PRJNA370163 | ENA

Escherichia coli strain:BW25113 JME57

Project description:Escherichia coli BW25113 JME57 resequencing genome sequencing

| PRJNA370164 | ENA

Escherichia coli strain:BW25113 JME58

Project description:Escherichia coli BW25113 JME58 resequencing genome sequencing

| PRJNA370165 | ENA

Escherichia coli strain:BW25113 JME61

Project description:Escherichia coli BW25113 JME61 resequencing genome sequencing

| PRJNA370166 | ENA

Escherichia coli strain:BW25113 JME74

Project description:Escherichia coli BW25113 JME74 resequencing genome sequencing

| PRJNA370041 | ENA

Escherichia coli strain:BW25113 JME80

Project description:Escherichia coli BW25113 JME80 resequencing genome sequencing

| PRJNA370045 | ENA

Escherichia coli strain:BW25113 JME72

Project description:Escherichia coli BW25113 JME72 resequencing genome sequencing

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Escherichia coli strain:BW25113 JME73

Project description:Escherichia coli BW25113 JME73 resequencing genome sequencing

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