Project description:Identification of putative proteins of interest that are involved in cathodic electron uptake by the novel iron-corroding strain D. ferrophilus IS5
Project description:Desulfovibrio ferrophilus IS5 was incubated on indium-tin oxide (ITO) electrodes poised at −0.4 V and −0.5 V (versus standard hydrogen electrode) in H-type reactors for 10 days. Total RNA was extracted from cells after incubation, and RNA fragments were purified and transcribed into cDNA. cDNA was sequenced by NovaSeq 6000 System.
Project description:Microbiologically influenced corrosion (MIC) is recognized as a considerable threat to carbon steel asset integrity in the oil and gas industry. There is an immediate need for reliable and broadly applicable methods for detection and monitoring of MIC. Proteins associated with microbial metabolisms involved in MIC could serve as useful biomarkers for MIC diagnosis and monitoring. A proteomic study was conducted using a lithotrophically-grown bacteria Desulfovibrio ferrophilus strain IS5, which is known to cause severe electric MIC in seawater environments. Unique proteins, which are differentially and uniquely expressed during severe microbial corrosion by strain IS5, were identified. This includes the detection of a multi-heme cytochrome protein predicted to be involved in extracellular electron transfer in the presence of the carbon steel. Thus, we conclude that newly identified protein biomarker for MIC could be used to generate easy-to-implement immunoassays for reliable detection of microbiological corrosion in the field.
Project description:RNA-sequencing (transcriptome analysis) of Desulfovibrio ferrophilus IS5 conducting electron uptake from electrodes at different potentials
