Project description:Here, we report the high-throughput profiling of histone modification (H3K9me2) in fission yeast Schizosaccharomyces pombe. We generated genome-wide H3K9me2 maps of fission yeast mutants in swo1-26 (temperature sensitive, ts) cells at 25℃ and 37℃. We find that H3K9me2 enrichment at heterochromatin regions, especially at the mating-type locus and subtelomeres, is compromised, suggesting heterochromatin assembly defects.
Project description:Screening for changes in the growth rate of heterozygous deletion diploid strains has been used in budding yeast to identify potentially rate-limiting steps for cellular growth. Using a similar methodology we examined the growth rates in rich medium for 4,334 fission yeast heterozygous deletion diploids.