ABSTRACT: Analysis of transgenerational effects on DNA copy number aberrations in male mice exposed to continuous 20mGy/day gamma-rays for 400 days (Secondary screening for 20mGyD familly).
Project description:Transgenerational effects of continuous low dose-rate (LDR) gamma-ray irradiation have not been well studied. Recent advances in DNA technology enabled us to examine a whole genome at molecular level. Here we adopted one of these techniques called oligo-microarray comparative genome hybridization (CGH) and studied trans-generational effects on DNA copy number aberrations (CNAs). C57BL/6JNrs male mice were exposed to LDR gamma-rays (0.05 mGy/day) for 400 days (total dose: 20 mGy) from 8 weeks of age. Progeny from 0.05 mGy/day irradiated mice had significantly lower frequencies of genomic aberrations than the progeny of non-irradiated mice.This study investigated the De novo mutation by comparing the parents and children using 15 LDR gamma-rays (0.05 mGy/day) irradiated male family and 25 non irradiated male family. This is a secondary screening. This study was performed under contract with the Aomori Prefectural Government, Japan.
Project description:Transgenerational effects of continuous low dose-rate (LDR) gamma-ray irradiation have not been well studied. Recent advances in DNA technology enabled us to examine a whole genome at molecular level. Here we adopted one of these techniques called oligo-microarray comparative genome hybridization (CGH) and studied trans-generational effects on DNA copy number aberrations (CNAs). C57BL/6JNrs male mice were exposed to LDR gamma-rays (0.05 mGy/day) for 400 days (total dose: 20 mGy) from 8 weeks of age. Progeny from 0.05 mGy/day irradiated mice had significantly lower frequencies of genomic aberrations than the progeny of non-irradiated mice.This study investigated the De novo mutation by comparing the parents and children using 15 LDR gamma-rays (0.05 mGy/day) irradiated male family and 25 non irradiated male family. This is a secondary screening. This study was performed under contract with the Aomori Prefectural Government, Japan.
Project description:Transgenerational effects of continuous low dose-rate (LDR) gamma-ray irradiation have not been well studied. Recent advances in DNA technology enabled us to examine a whole genome at molecular level. Here we adopted one of these techniques called oligo-microarray comparative genome hybridization (CGH) and studied trans-generational effects on DNA copy number aberrations (CNAs). C57BL/6JNrs male mice were exposed to LDR gamma-rays (0.05 mGy/day) for 400 days (total dose: 20 mGy) from 8 weeks of age. Progeny from 0.05 mGy/day irradiated mice had significantly lower frequencies of genomic aberrations than the progeny of non-irradiated mice.This study investigated the De novo mutation by comparing the parents and children using 15 LDR gamma-rays (0.05 mGy/day) irradiated male family and 25 non irradiated male family. This is a secondary screening. This study was performed under contract with the Aomori Prefectural Government, Japan.
Project description:Transgenerational effects of continuous low dose-rate (LDR) gamma-ray irradiation have not been well studied. Recent advances in DNA technology enabled us to examine a whole genome at molecular level. Here we adopted one of these techniques called oligo-microarray comparative genome hybridization (CGH) and studied trans-generational effects on DNA copy number aberrations (CNAs). C57BL/6JNrs male mice were exposed to LDR gamma-rays (0.05 mGy/day) for 400 days (total dose: 20 mGy) from 8 weeks of age. Progeny from 0.05 mGy/day irradiated mice had significantly lower frequencies of genomic aberrations than the progeny of non-irradiated mice.This study investigated the De novo mutation by comparing the parents and children using 15 LDR gamma-rays (0.05 mGy/day) irradiated male family and 25 non irradiated male family. This is a secondary screening. This study was performed under contract with the Aomori Prefectural Government, Japan.
Project description:Transgenerational effects of continuous low dose-rate (LDR) gamma-ray irradiation have not been well studied. Recent advances in DNA technology enabled us to examine a whole genome at molecular level. Here we adopted one of these techniques called oligo-microarray comparative genome hybridization (CGH) and studied trans-generational effects on DNA copy number aberrations (CNAs). C57BL/6JNrs male mice were exposed to LDR gamma-rays (0.05 mGy/day) for 400 days (total dose: 20 mGy) from 8 weeks of age. Progeny from 0.05 mGy/day irradiated mice had significantly lower frequencies of genomic aberrations than the progeny of non-irradiated mice.This study investigated the De novo mutation by comparing the parents and children using 15 LDR gamma-rays (0.05 mGy/day) irradiated male family and 25 non irradiated male family. This is a secondary screening. This study was performed under contract with the Aomori Prefectural Government, Japan.
Project description:Transgenerational effects of continuous low dose-rate (LDR) gamma-ray irradiation have not been well studied. Recent advances in DNA technology enabled us to examine a whole genome at molecular level. Here we adopted one of these techniques called oligo-microarray comparative genome hybridization (CGH) and studied trans-generational effects on DNA copy number aberrations (CNAs). C57BL/6JNrs male mice were exposed to LDR gamma-rays (0.05 mGy/day) for 400 days (total dose: 20 mGy) from 8 weeks of age. Progeny from 0.05 mGy/day irradiated mice had significantly lower frequencies of genomic aberrations than the progeny of non-irradiated mice.This study investigated the De novo mutation by comparing the parents and children using 15 LDR gamma-rays (0.05 mGy/day) irradiated male family and 25 non irradiated male family. This is a secondary screening. This study was performed under contract with the Aomori Prefectural Government, Japan.
Project description:Transgenerational effects of continuous low dose-rate (LDR) gamma-ray irradiation have not been well studied. Recent advances in DNA technology enabled us to examine a whole genome at molecular level. Here we adopted one of these techniques called oligo-microarray comparative genome hybridization (CGH) and studied trans-generational effects on DNA copy number aberrations (CNAs). C57BL/6JNrs male mice were exposed to LDR gamma-rays (0.05 mGy/day) for 400 days (total dose: 20 mGy) from 8 weeks of age. Progeny from 0.05 mGy/day irradiated mice had significantly lower frequencies of genomic aberrations than the progeny of non-irradiated mice.This study investigated the De novo mutation by comparing the parents and children using 15 LDR gamma-rays (0.05 mGy/day) irradiated male family and 25 non irradiated male family. This is a secondary screening. This study was performed under contract with the Aomori Prefectural Government, Japan.
Project description:Transgenerational effects of continuous low dose-rate (LDR) gamma-ray irradiation have not been well studied. Recent advances in DNA technology enabled us to examine a whole genome at molecular level. Here we adopted one of these techniques called oligo-microarray comparative genome hybridization (CGH) and studied trans-generational effects on DNA copy number aberrations (CNAs). C57BL/6JNrs male mice were exposed to LDR gamma-rays (0.05 mGy/day) for 400 days (total dose: 20 mGy) from 8 weeks of age. Progeny from 0.05 mGy/day irradiated mice had significantly lower frequencies of genomic aberrations than the progeny of non-irradiated mice.This study investigated the De novo mutation by comparing the parents and children using 15 LDR gamma-rays (0.05 mGy/day) irradiated male family and 25 non irradiated male family. This is a secondary screening. This study was performed under contract with the Aomori Prefectural Government, Japan.
Project description:Transgenerational effects of continuous low dose-rate (LDR) gamma-ray irradiation have not been well studied. Recent advances in DNA technology enabled us to examine a whole genome at molecular level. Here we adopted one of these techniques called oligo-microarray comparative genome hybridization (CGH) and studied trans-generational effects on DNA copy number aberrations (CNAs). C57BL/6JNrs male mice were exposed to LDR gamma-rays (0.05 mGy/day) for 400 days (total dose: 20 mGy) from 8 weeks of age. Progeny from 0.05 mGy/day irradiated mice had significantly lower frequencies of genomic aberrations than the progeny of non-irradiated mice.This study investigated the De novo mutation by comparing the parents and children using 15 LDR gamma-rays (0.05 mGy/day) irradiated male family and 25 non irradiated male family. This is a secondary screening. This study was performed under contract with the Aomori Prefectural Government, Japan.
Project description:Transgenerational effects of continuous low dose-rate (LDR) gamma-ray irradiation have not been well studied. Recent advances in DNA technology enabled us to examine a whole genome at molecular level. Here we adopted one of these techniques called oligo-microarray comparative genome hybridization (CGH) and studied trans-generational effects on DNA copy number aberrations (CNAs). C57BL/6JNrs male mice were exposed to LDR gamma-rays (0.05 mGy/day) for 400 days (total dose: 20 mGy) from 8 weeks of age. Progeny from 0.05 mGy/day irradiated mice had significantly lower frequencies of genomic aberrations than the progeny of non-irradiated mice.This study investigated the De novo mutation by comparing the parents and children using 15 LDR gamma-rays (0.05 mGy/day) irradiated male family and 25 non irradiated male family. This is a secondary screening. This study was performed under contract with the Aomori Prefectural Government, Japan.
