Project description:Cleome gynandra leaf development RNA-seq RNA-seq have been performed on developing (2mm length) and mature (10mm length) leaves of the C4 photosynthesis plant Cleome gynandra. 6 runs of Illumina 36-42 bp single end have been performed ArrayExpress Release Date: 2010-12-01 Publication Author List: Sylvain Aubry, Mali Salmon, Kim M Rutherford, Paul Bertone, Andrea Brautigam, Andreas PM Weber, Krystyna A Kelly, Julian M Hibberd Publication Title: De novo transcriptome assembly enables quantitative expression analysis in non-sequenced model organisms Person Roles: submitter Person Last Name: Aubry Person First Name: Sylvain Person Mid Initials: Person Email: sa530@cam.ac.uk Person Phone: +441223 330 220 Person Address: Department of Plant Sciences. University of Cambridge. Downing Street. CB2 3EA Cambridge Person Affiliation: University of Cambridge
Project description:RNA-seq have been performed on developing (2mm length) and mature (10mm length) leaves of the C4 photosynthesis plant Cleome gynandra. 6 runs of Illumina 36-42 bp single end have been performed
Project description:We have sequenced the whole genome of Cleome hassleriana by NGS [SRA accession number SRA058749] and build a reference sequence for this genome. In order to improve quality of gene models, a mix transcriptome sample extracted from the bud, leaf, petiole, stems and flowers of Cleome. A mixed RNA pool extracted from the tissues of bud, leaf, petiole, stems and flower is sequenced for Cleome hassleriana.
Project description:We have sequenced the whole genome of Cleome hassleriana by NGS [SRA accession number SRA058749] and build a reference sequence for this genome. In order to improve quality of gene models, a mix transcriptome sample extracted from the bud, leaf, petiole, stems and flowers of Cleome.
Project description:We isolated and compared transcriptomes of GC and M from C3 T. hassleriana and C4 G. gynandra. This was achieved using laser-capture microdissection of each cell type from fixed paradermal sections. At least 2,500 cells were isolated for each replicate. RNA was extracted, amplified and subjected to RNAseq.
Project description:In Cleomaceae species, NAD-malic enzyme (NAD-ME) was independently co-opted to participate in C4 photosynthesis. In C4 Cleome species, all NAD-ME genes (NAD-MEα, -ß1 and -ß2) were affected by C4 evolution and are expressed at higher levels than their C3 orthologs in Tarenaya hassleriana. In C3 Cleome, the NAD-ME housekeeping function is performed by two heteromers, NAD-MEα/ß1 and NAD-MEα/ß2, with similar biochemical properties and tissue occurrence. In the C4 species, Gynandropsis gynandra and Cleome angustifolia, this role is performed only by the NAD-MEα/ß2 heteromer. In these C4 species NAD-MEα/ß1 is preferentially present in leaves of the C4 species where is the predominant isoform. GgNAD-MEα/ß1 exhibits high catalytic efficiency and is differentially activated by the C4 intermediate aspartate. In C4 Cleome NAD-MEα/ß1 represents thus the C4-decarboxylase. GgNAD-MEß1and CaNAD-MEß1 are non-catalytic subunits but impart a stabilizing effect on the associated α-subunit. We conclude that in C4 Cleome the functions of NAD-ME as a TCA cycle associated enzyme and as a C4 photosynthetic decarboxylase coexist in BSC mitochondria and are performed by isoforms originated through associations of differentially adapted subunits.
Project description:Assembly of the plant OXPHOS complexes was studied using complexome profiling. mitochondria were extracted from seedlings or mature leaves of the reference organism Arabidopsis thaliana. In seedlings, a fast-growing tissue, mitochondria biogenesis is very active whereas in mature leaves, little biogenesis is occurring. Comparison of both datasets allow the identification of assembly intermediates that are accumulating in seedlings but not in leaves.
