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Investigating Human Placentation and Pregnancy Using First Trimester Chorionic Villi

ABSTRACT: Investigating Human Placentation and Pregnancy Using First Trimester Chorionic Villi

PROVIDER: PRJNA377795 | ENA |

REPOSITORIES: ENA

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Investigating Human Placentation and Pregnancy Using First Trimester Chorionic Villi

Project description:<p>Chorionic villus sampling (CVS) is routinely used for prenatal diagnosis of cytogenetic disorders, but also possesses great potential for studying placentation. To better understand villi biology, human placentation, and how these relate to pregnancy outcomes, we examined the morphology and transcriptomes of villi obtained via CVS from 10-14 weeks of pregnancy and correlated these with pregnancy attributes and clinical outcomes.</p>

| phs001320 | dbGaP

Chorionic villi cells

2007-02-17 | GSE7021 | GEO

Transcription profiling of human chorionic villi cells co-culutured with fetal cardiomyocytes

Project description:In the chorionic villi of placenta, trophoblasts and endothelial cells are present, and moreover mesenchymal cells (stromal cells) can be obtained. We generated cells with the mesenchymal phenotype from the chorionic mesoderm, and showed that: a) physiologically functioning cardiomyocytes were transdifferentiated from human placenta-derived chorionic villi cells, but these cells did not induce to osteoblasts and adipocytes ; b) the cardiomyogenic induction rate obtained using our system was relatively high compared to that obtained using the previously described method ; c) co-cultivation with fetal murine cardiomyocytes alone without transdifferentiation factors such as 5-azaC or oxytocin is sufficient for cardiomyogenesis in our system; d) Chorionic villi cells have the electrophysiological properties of 'working' cardiomyocytes. The chorionic mesoderm contained a large number of cells with a cardiomyogenic potential. Experiment Overall Design: To isolate chorionic villi cells, we used the explant culture method, in which the cells were outgrown from pieces of chorionic villi attached to dishes. Chorionic villi cells were harvested with 0.25% trypsin and 1 mM EDTA, and overlaid onto the cultured fetal cardiomyocytes at 7 x 103/cm2. Every 2 days, the culture medium was replaced with fresh culture medium that was supplemented with 10% FBS and 1 ug/ml Amphotericin B (GIBCO). The morphology of the beating chorionic villi cells was evaluated under a fluorescent microscope.

2008-06-15 | E-GEOD-7021 | biostudies-arrayexpress

Characteristic DNA methylation profiles of chorionic villi in recurrent miscarriage

Project description:Genome wide DNA methylation profiling of chorionic villi and decidua from recurrent miscarriage patients and artificial abortion controls. Infinium HumanMethylation450 BeadChip was used.

2022-04-24 | GSE198700 | GEO

Homo sapiens

Project description:Investigating Human Placentation and Pregnancy Using First Trimester Chorionic Villi

| PRJNA377796 | ENA

Prevalence of chromosomal alterations in first-trimester spontaneous pregnancy loss

Project description:Pregnancy loss is often caused by chromosomal abnormalities of the conceptus. The prevalence of these abnormalities and the allocation of (ab)normal cells in embryonic and placental lineages during intrauterine development remain elusive. We analyzed 1,745 spontaneous pregnancy losses and found that roughly half (50.4%) of the products of conception (POC) were karyotypically abnormal, with maternal and paternal age independently contributing to the increased genomic aberration rate in pregnancy loss. We applied genome haplarithmisis to a subset of 94 pregnancy losses with normal parental and POC karyotypes. Genotyping of parental DNA as well as POC extraembryonic mesoderm and chorionic villi DNA, representing embryonic and trophoblastic tissues, enabled characterization of the genomic landscape of both lineages. Of these pregnancy losses, 35.1% had chromosomal aberrations not previously detected by karyotyping, increasing the rate of aberrations of pregnancy losses to 67.8% by extrapolation. In contrast to viable pregnancies where mosaic chromosomal abnormalities are often restricted to chorionic villi, such as confined placental mosaicism, we found a higher degree of mosaic chromosomal imbalances in extraembryonic mesoderm rather than chorionic villi in pregnancy losses. Our results stress the importance of scrutinizing the full allelic architecture of genomic abnormalities in pregnancy loss to improve clinical management and basic research of this devastating condition.

2023-10-05 | GSE228151 | GEO

Expression profile of micro-RNAs and functional annotation analysis of their targets in human chorionic villi from early recurrent miscarriage

Project description:This study was designed to investigate the miRNA expression profilein human chorionic villi from early recurrent miscarriage. We identified 155 miRNAs exhibiting over 2-fold change in expression levels (P<0.05) by microarray,

2015-12-31 | GSE73025 | GEO

Characteristic DNA methylation profiles of chorionic villi in recurrent miscarriage

Project description:Characteristic DNA methylation profiles of chorionic villi in recurrent miscarriage

| PRJNA816500 | ENA

Human first-trimester chorionic villi have a myogenic potential.

Project description: Not available

| S-EPMC3316778 | biostudies-literature

RNA-seq of first-trimester chorionic villus for understanding the maintenance of early gestation as well as pathogenesis in pregnancy-related disorders

Project description:Implant failure and insufficient placental development are important causes of female infertility, recurrent miscarriage, and other pregnancy-related problems. A better understanding of gene expression profiling based on high-throughput sequencing technology is important for the study on the development of the placenta and the causes of pregnancy-related diseases. In this study, we collected 6 first-trimester chorionic villus and decidua tissues and obtained the transcriptome database on high-throughput sequencing. We performed routine principal component analysis (PCA) on these 12 samples and identified differentially expressed genes from samples with different sex background. And identified genes highly expressed in villus and decidua, respectively.

2019-08-31 | E-MTAB-8186 | biostudies-arrayexpress

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