Project description:Aim: compare the transcriptionnal responses induced by mycorrhiza in wild type and GRAS transcription factor RAM1 mutant ram1-2 in roots. Results: 650 genes were upregulated in mycorrhized WT (fold change >5; FDR<0,05, experiment difference>10) and 201 in the ram1 mutant. 182 genes were induced in both WT and ram1. 468 and 19 genes were induced only in WT or ram1-2 respectively. 91 genes were downregulated in mycorrhized WT (fold change <5; FDR<0,05, experiment difference>10) and 51 in ram1-2. There was no overlap in the downregulated genes. Conclusion: The transcriptionnal response to mycorrhiza is more important in WT than in ram1-2. Gene induction in ram1 mostly overlap with wild type. Our results confirm that ram1 plays a central role in the root response to mycorrhization.
Project description:Investigation of gene expression level changes in Petunia hybrida seedlings subjected to cold at 2°C for 0.5 h, 2 h, 24 h and 5 d, compared to the CK.
