Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA381258 | ENA |
REPOSITORIES: ENA
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Project description:Biological insights from exome sequences of human embryonic stem cell lines
| PRJNA381257 | ENA
Project description:<p>Human embryonic stem cells (hESCs) are a powerful tool for the study of human development and can form the basis of cellular disease models or therapies. However, the genetic make-up and stability of hESCs has not been systematically studied at a genome-wide level with single nucleotide resolution. We therefore sequenced the whole exomes of widely available hESCs. The data generated has provided new insights about the nature of acquired variation and the genomic integrity of the cell lines. We anticipate that this online resource will enable investigators to access raw sequencing data in order to interrogate cell lines for different disease and trait-associated genetic variants.</p>
| phs001343 | dbGaP
Project description:<p>In this study we used next generation deep sequencing technologies to analyze the genomes of Harvard University Stem Cell lines 63 and 64. We performed 101-bp paired-end whole genome sequencing of the two cell lines using Illumina HiSeq platforms. The sequence reads obtained were analyzed for copy number and used for replication timing analysis. Our data suggests that read depth profiles can be used to map replication timing in Embryonic Stem Cells (ESCs). Further we observe that replication profiles are highly correlated across ESCs but distinct from those of other cell types such as Lymphoblastoid Cell Lines (LCLs). These results demonstrated that read depth data from whole genome sequencing can be used to study variation in replication timing within the human population and across different cell types. Whole genome sequences from HUES63 and HUES64 used for this study are being submitted.</p>
| phs000825 | dbGaP
Project description:Affymetrix SNP 6.0 array data for human embryonic stem cell lines
| PRJNA123109 | ENA
Project description:Identification and Characterization of Subpopulations within Human Embryonic Stem Cell Lines
| PRJNA132669 | ENA
Project description:Comparative genomic hybridization of human embryonic stem cell lines deposited in the National Stem Cell Bank
| PRJNA102971 | ENA
Project description:Metabolism is vital to cellular function and tissue homeostasis during human lung development. In utero, embryonic pluripotent stem cells undergo endodermal differentiation towards a lung progenitor cell fate that can be mimicked in vitro using induced human pluripotent stem cells (hiPSCs) to study genetic mutations. To identify differences between wild type and surfactant protein B (SFTPB)-deficient cell lines during endoderm specification towards lung, we used an untargeted metabolomics approach to evaluate the developmental changes in metabolites. We found that the metabolites most enriched during the differentiation from pluripotent stem cell to lung progenitor cell, regardless of cell line, were sphingomyelins and phosphatidylcholines, two important lipid classes in fetal lung development. The SFTPB mutation had no metabolic impact on early endodermal lung development. The identified metabolite signatures during lung progenitor cell differentiation may be utilized as biomarkers for normal embryonic lung development.
2021-06-29 | ST002055 | MetabolomicsWorkbench
Project description:Molecular karyotype analysis of 25 clinical-grade human embryonic stem cell lines
| PRJNA282945 | ENA
Project description:Transcriptome variation among human embryonic stem cell lines impacts on their differentiation
| PRJNA397398 | ENA