Genomics

Dataset Information

0

Synthetic construct


ABSTRACT: An arrayed CRISPR library for individual, combinatorial and multiplexed gene knockout I

ORGANISM(S): synthetic construct  

PROVIDER: PRJNA381772 | ENA |

REPOSITORIES: ENA

Dataset's files

Source:
Action DRS
SRR5419495.fastq.gz Fastqsanger.gz
SRR5419496.fastq.gz Fastqsanger.gz
SRR5419497.fastq.gz Fastqsanger.gz
SRR5419498.fastq.gz Fastqsanger.gz
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Publications

A CRISPR Resource for Individual, Combinatorial, or Multiplexed Gene Knockout.

Erard Nicolas N   Knott Simon R V SRV   Hannon Gregory J GJ  

Molecular cell 20170701 2


We have combined a machine-learning approach with other strategies to optimize knockout efficiency with the CRISPR/Cas9 system. In addition, we have developed a multiplexed sgRNA expression strategy that promotes the functional ablation of single genes and allows for combinatorial targeting. These strategies have been combined to design and construct a genome-wide, sequence-verified, arrayed CRISPR library. This resource allows single-target or combinatorial genetic screens to be carried out at  ...[more]

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