ABSTRACT: RA-MAP: mapping molecular immunological landscapes in early rheumatoid arthritis and healthy vaccine recipients [Healthy Hepatitis B Vaccine Recipients]
Project description:<p>Rheumatoid arthritis (RA) is a chronic inflammatory disorder with poorly defined aetiology characterised by synovial inflammation with variable disease severity and drug responsiveness. To investigate the peripheral blood immune cell landscape of RA, we performed comprehensive clinical and molecular profiling of 267 RA patients and 52 healthy vaccine recipients for up to 18 months to establish a high quality sample biobank including plasma, serum, peripheral blood cells, urine, genomic DNA, RNA from whole blood, lymphocyte and monocyte subsets. We have performed extensive multi-omic immune phenotyping, including genomic, metabolomic, proteomic, transcriptomic and autoantibody profiling. We anticipate that these detailed clinical and molecular data will serve as a fundamental resource offering insights into immune-mediated disease pathogenesis, progression and therapeutic response, ultimately contributing to the development and application of targeted therapies for RA.</p>
Project description:miRNA differential expression in Rheumatoid arthritis(RA) blood: Microarray analysis of miRNAs in blood from RA and healthy donors.
Project description:Gene expression profiling of peripheral blood cells from patients with rheumatoid arthritis (RA)/ systemic lupus erythematosus (SLE)/ polyarticular type juvenile idiopathic arthritis (polyJIA)/ systemic-onset JIA (sJIA) vs healthy children (HC) and healthy individual (HI).
Project description:Rheumatoid arthritis (RA) is a systemic autoimmune disease and its underlying molecular mechanisms are still poorly understood. Previously a CD4 T-cell microarray study has only focused arthritis patients. We aimed to compare the molecular profiles of active RA versus healthy control in CD4 T cells. We used microarrays to detail the global programme of gene expression in active RA and healthy control.
Project description:Genome wide microRNA profiling in peripheral blood mononuclear cells from rheumatoid arthritis (RA) patients and healthy controls. The Affymetrix miRNA 4.0 chip was used to obtain RNA profiles in 28 RA patients and 18 healthy controls.
Project description:In order to identify new biomarkers for the diagnosis of rheumatoid arthritis, we used circRNA microarray technology to screen the differential expression of circRNA in peripheral blood mononuclear cells of patients with rheumatoid arthritis. We identified a total of 399 differentially expressed circRNAs in RA patients and healthy controls, of which 149 circRNAs were significantly up-regulated in RA patients and 250 were down-regulated. Among them, hsa_circRNA_101328 may be a potential biomarker for the diagnosis of RA.
Project description:A total of 762 proteins were identified from 35 pooled rheumatoid arthritis (RA) patients with moderate to high disease activity and 60 pooled healthy serum samples. Among them, 142 proteins including 68 up-regulation and 74 down-regulation were identified in RA compared to healthy subjects.
