Genomics

Dataset Information

0

Homo sapiens


ABSTRACT: Human telomerase requires TCAB1 for catalytic function and for shaping RNA component conformation

PROVIDER: PRJNA381961 | ENA |

REPOSITORIES: ENA

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Publications

Analysis of RNA conformation in endogenously assembled RNPs by icSHAPE.

Chen Lu L   Chang Howard Y HY   Artandi Steven E SE  

STAR protocols 20210428 2


The majority of the mammalian genome is transcribed into non-coding RNAs, many of which co-evolve with RNA-binding proteins (RBPs) to function as biochemically defined and tractable ribonucleoproteins (RNPs). Here, we applied icSHAPE- a robust and versatile RNA structural probing pipeline- to endogenous RNPs purified from nuclei, providing base-resolution structural rationale for RNP activity and subcellular localization. Combining with genetic and biochemical reconstitutions, structural and fun  ...[more]

Publication: 1/2

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