Project description:The expression of the long non-coding RNAs (lncRNAs) in the active tuberculosis patients,multidrug-resistant tuberculosis patients,cured tuberculosis subjects and healthy controls were scanned using lncRNA microarray.The Arraystar Human LncRNA Microarray version 4.0 (Agilent Technologies) was used,containing 40173 lncRNA probes and 20730 mRNA probes for the detection of lncRNAs and mRNAs in the sample. Non-coding RNAs and mRNAs profiling by array
Project description:Dysregulated expression of long noncoding RNAs (lncRNAs) has been demonstrated as being implicated in a variety of human diseases. In the study we aimed to determine lncRNA profile in CD8+ T cells response to active tuberculosis (TB).
Project description:Although many protein-coding genes have been identified to be aberrantly expressed in cervical cancer, the mechanisms of development and progression of cervical cancer remain unclear. In recent years, non-coding RNAs, especially including microRNAs and long non-coding RNAs, have been shown to play important regulatory roles in mammalian cell biology. In our study, we investigated the whole genome gene expression level changes by human transcriptome array in tumor tissues and paired adjacent non-tumor tissue of patients with cervical cancer. The functions of different expression microRNAs, long non-coding RNAs and mRNAs were further analyzed in vitro and in vivo using loss-of-function and gain-of-function approaches.
Project description:Although many protein-coding genes have been identified to be aberrantly expressed in cervical cancer, the mechanisms of development and progression of cervical cancer remain unclear. In recent years, non-coding RNAs, especially including microRNAs and long non-coding RNAs, have been shown to play important regulatory roles in mammalian cell biology. In our study, we investigated the whole genome gene expression level changes by human transcriptome array in tumor tissues and paired adjacent non-tumor tissue of patients with cervical cancer. The functions of different expression microRNAs, long non-coding RNAs and mRNAs were further analyzed in vitro and in vivo using loss-of-function and gain-of-function approaches. A ten chip study using total RNA recovered from five separate cervical cancer tissues and five paired adjacent non-tumor samples.
Project description:Glioblastoma multiforme(GBM) is the most common and lethal malignant primary brain tumor. Long non-coding RNAs (lncRNAs) are aberrantly expressed in many cancers and are involved in their cell proliferation, apoptosis, angiogenesis, and invasion. To systematically study the role of lncRNAs in GBM, we built gene expression profiles of GBM and non-tumor tissues using lncRNA and mRNA gene expression microarrays.
