Project description:We generated 120 Gb of raw sequencing data (~10 Gb per sample) and catalogued the expression profiles of 55106 annotated unigenes.We identified numerous differentially expressed genes that exhibited distinct expression patterns, and tight junction that were highly differentiated between S40 and S45, as well as between S36 and S43, S45. And genes related to glycolysis and pancreatic secretion were significantly enrichement beweent S43 and S45, these genes have known or potential roles in defense response and were enriched in functional gene categories potentially responsible for cucumber resistance.These results indicated that there are two process during metamorphosis, first one from S36-S43 is the larvel muscle development and apopsist, and the second from S43-S45 is the adult muscle development. Therefore, they are appealing candidates for further investigation of the gene expression and associated regulatory mechanisms related to muscle remodeling during metamorphosis.
Project description:The sublethal effects of a practically nontoxic GBH (KISSUN®) were investigated in tadpoles of M. fissipes, a farmland inhabiting amphibian. GBH exposure caused higher temperature preference, growth suppression, shorter and weaker tails in these tadpoles. These sublethal effects would likely affect the environmental adaptation ability, locomotor activity, competitive capacity and survival rate of tadpoles and thus might have profound influence on their population dynamics. Higher temperature preference might be an adaptive strategy for improving the efficiency of damage repair system activated by GBH exposure, as well as compensating for the reduced locomotor activity and sustaining energy metabolism. Growth suppression and tail morphological change were associated with reduced intensity of overall protein synthesis and structural protein synthesis, especially muscle specific genes. Transcriptional analysis suggested that GBH-treated tadpoles had suppressed carbohydrate and lipid catabolism, while encouraged amino acid catabolism. We speculated that metabolic switch to amino acid might be an adaptive strategy for compensating the reduced contribution of carbohydrate and lipid in energy production, and it provide a metabolic explanation for the downregulation of protein synthesis, growth synthesis and morphological change. Our results systemically revealed the molecular processes underlying the sublethal effects in an amphibian caused by GBH. It suggested that metabolic switch might be a consensus toxicologic mechanism of growth suppression across fish and amphibians.
