Project description:Transcriptional profiling to investigate the regulatory roles of SpxA and SpxB of S. mutans. RNA was extracted from four replicate samples of each strain of interest (spxA mutant, spxB mutant, spxAB double-mutant, UA159 wild type) and labeled with Cy3. For each replicate, labeled RNA was hybridized to slides along with Cy5-labeled reference RNA, extracted from S. mutans UA159 cells grown to mid-log.
Project description:Transcriptional profiling of early logarithmic phase culture (O.D=0.2-0.3) of Streptococcus mutans UA159 comparing control of untreated Streptococcus mutans UA159 bacteria with Streptococcus mutans UA159 bacteria spplemented with 20µM synthetic DPD (pre-AI-2) which regulates gene expression via AI-2 quorum sensing system.Three compairisons were performed at pHs of 7,6 and 5.
Project description:Transcriptional analysis of glucose shock vs. steady-state growth in the parent strain and an acid sensitive mutant strain of S. mutans
Project description:Transcriptional comparisons between wild-type Streptococcus mutans UA159 and two mutant strains (M-bM-^HM-^FackA and M-bM-^HM-^FackA pta). On growth condition in defined FMC medium, WT vs. either M-bM-^HM-^FackA or M-bM-^HM-^FackA pta mutants. Biological replicates: 10 control, 5 each mutant, independently grown and harvested. One replicate per array.
