Genomics

Dataset Information

235

Homo sapiens


ABSTRACT: mRNA Sequencing of Human PromoCells Using 3'-directed Digital Gene Expression (3'-DGE) Technique

ORGANISM(S): Homo sapiens  

PROVIDER: PRJNA385094 | ENA |

REPOSITORIES: ENA

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Publications


Creating a cDNA library for deep mRNA sequencing (mRNAseq) is generally done by random priming, creating multiple sequencing fragments along each transcript. A 3'-end-focused library approach cannot detect differential splicing, but has potentially higher throughput at a lower cost, along with the ability to improve quantification by using transcript molecule counting with unique molecular identifiers (UMI) that correct PCR bias. Here, we compare an implementation of such a 3'-digital gene expre  ...[more]

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