Project description:The mechanisms of cellular and molecular adaptation of fungi to salinity have been commonly drawn from halotolerant strains, although some exceptions in basidiomycete fungi can be found. These studies have been conducted in settings where cells are subjected to stress, either hypo or hyperosmotic, which can be a confounding factor in describing physiological mechanisms related to salinity. Here, we have studied transcriptomic changes in Aspergillus sydowii, a halophilic species, when growing in three different salinity conditions (No salt, 0.5M and 2.0M NaCl). In this fungus salinity related responses occur under high salinity (2.0M NaCl) and not when cultured under optimal conditions (0.5M NaCl), suggesting that in this species, most of the mechanisms described for halophilic growth are a consequence of saline stress response and not an adaptation to saline conditions.
Project description:Aspergillus sydowii was grown under low and high-salt conditions. Cell-free extracts were recovered, dried-down and metabolites extracted using cold acetone. Metabolites were separated by liquid chromatography and data was acquired (data-dependent acquisition) on a Sciex TripleTOF 5600 under positive ion mode. The accumulation time for TOF MS was 0.25 s/spectra over the m/z range 100-1500 Da and for MS/MS scan was 0.05 s/spectra over the m/z 50-1500 Da.
