Project description:Ascertain the transcriptional response of the major malaria vector Anopheles coluzzii to the pyrethroid deltamethrin over ten time points.
Project description:Malaria control relies on insecticides targeting the mosquito vector, but is being increasingly compromised by insecticide resistance. Elevated expression of metabolic enzymes frequently drives resistance. In diploids, gene expression is regulated both in cis, by regulatory sequences on the same chromosome, and by trans acting factors, affecting both alleles equally. Differing levels of transcription can be caused by mutations in cis-regulatory modules, but few cis-regulatory modules controlling the expression of genes that determine insecticide resistance have been identified. Genes potentially under differential cis-regulation between bendiocarb resistant and susceptible Anopheles gambiae strains were identified by counting transcripts produced from maternal and paternal alleles in F1 hybrids of these strains (allelic specific expression). Cis regulatory module sequences controlling gene expression in insecticide resistance relevant tissues such as midgut, Malpighian tubules and legs were predicted using a previously established machine learning method. These predictions included CRM proximal to both genes under differential cis regulation and genes that show consistent differential expression patterns in multiple resistant Anopheles strains.
Project description:Field resistant Anopheles coluzzii were compared to the lab susceptible Anopheles coluzzii N'Gousso. The samples were collected in 2014 in Burkina Faso and show resistance to pyrethroid insecticides.
Project description:Pseudomonas aeruginosa, the type species of the Pseudomonas genus, is an environmental Gram negative bacterium, well-known for its ability to produce toxins, resist antibiotics, and opportunistically colonize various niches, including invertebrate and vertebrate hosts. P. aeruginosa produces redox active secondary metabolites called phenazines involved in quorum sensing, biofilm formation, virulence, and iron acquisition. Moreover, these colorful pigmented virulence factors act as ligands for the highly conserved aryl hydrocarbon receptor (AhR) thereby regulating antibacterial defenses in vertebrates. Pseudomonas spp. are some of the most frequently identified bacteria in larval and adult stages of wild mosquito populations. Here we investigated global transcriptional changes induced in A. coluzzii third instar larvae incubated with a sublethal concentration (50 µM) of 1-hydroxyphenazine (1-HP) or pyocyanin (Pyo) at 4 h and 8 h of continuous incubation by whole-genome DNA microarrays.
