Project description:This a reciprocal transplant experiment. Two hebivorous lepidoptera species (Ostrinia nubilalis and Ostrinia scapulalis) have been fed either their preferred plant (corn for O.nubilalis and Mugwort for O. scapulalis) or the reciprocal plant. At 4th larval instar, RNA was extracted from whole larvae, size selected to enrich in small RNAs and sequenced by Illumina single-end 50bp.
Project description:The goals of this study are to obtain the transcriptome profiling (RNA-seq) of different developmental stages and tissues in Ostrinia furnacalis. Methods: O. furnacalis embryos were collected less than 12 h post oviposition. 1st, 3rd and 5th instar of the Asian corn borer, which represents the newly hatched larvae, the middle stage of larvae and the mature larvae, was harvested for subsequent experiments. Pupae and adults were grouped into females and males, then female and male samples were mixed at the ratio of 1:1. The 3rd instar larvae were anesthetized on an ice plate for tissue extraction. The midgut, fat body and silk gland were isolated. All these samples were generated by deep sequencing, in triplicate, using Illumina NovaSeq 6000. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. Results: We obtained the transcriptome profiling (RNA-seq) of different developmental stages and tissues in Ostrinia furnacalis Conclusions: Our study representsa detailed analysis of different developmental stages and tissues in Ostrinia furnacalis
