Ontology highlight
ABSTRACT:
OTHER RELATED OMICS DATASETS IN: PXD006708
PROVIDER: PRJNA390722 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:We assessed the off-target effects of M2 expression on the leaf proteome of Nicotiana benthamiana infiltrated with the bacterial gene vector Agrobacterium tumefaciens. A iTRAQ quantitative proteomics procedure was followed to compare the leaf proteomes of plants agroinfiltrated with either an ‘empty’ vector or an M2-encoding vector. Leaves infiltrated with the empty vector had a low soluble protein content compared to non-infiltrated control leaves, associated with increased levels of stress-related proteins but decreased levels of photosynthesis-associated proteins. M2 expression partly compromised these effects of agroinfiltration to restore soluble protein content in leaf tissue, associated with restored levels of photosynthesis-associated proteins and reduced levels of stress-related proteins in the apoplast. These data illustrate the cell-wide influence of Golgi lumen pH homeostasis on the leaf proteome of N. benthamiana responding to microbial challenge.
2019-12-06 | PXD014316 | Pride
Project description:⢠A comparison of the transcriptomes of russeted vs. waxy apple exocarps previously highlighted a tight relationship between a gene encoding a MYB-type transcription factor, MdMYB93, and some key suberin biosynthetic genes. The present work assesses the role of this transcription factor in the suberization process. ⢠A phylogenetic analysis of MdMYB93 and Arabidopsis thaliana MYBs was performed and the function of MdMYB93 was further investigated using Agrobacterium-mediated transient overexpression in Nicotiana benthamiana leaves. An RNA-Seq analysis was performed to highlight the MdMYB93-regulated genes. UPLC-TripleTOF and GC-MS were used to investigate alterations in phenylpropanoid, soluble free lipid, and lipid polyester contents. ⢠A massive accumulation of suberin and its biosynthetic precursors in MdMYB93 agro-infiltrated leaves was accompanied by a remobilization of phenylpropanoids and an increased amount of lignin precursors. Gene expression profiling displayed a concomitant alteration of lipid and phenylpropanoid metabolism, cell wall development, and extracellular transport, with a large number of induced transcripts predicted to be involved in suberin deposition. ⢠The present work supports a major role of MdMYB93 in the regulation of suberin deposition in russeted apple skins, from the synthesis of monomeric precursors, their transport, polymerization, and final deposition as suberin in primary cell wall. In order to draw a consistent picture of a gene expression profile of the MYB93 induced was performed comparing of 4 biological replicates of Nicotiana benthamiana leaves infiltrated with Pearleygate103 35S::MdMYB93 and 4 biological replicates of control plants infiltrated with P19 only.
2016-07-15 | E-GEOD-83618 | biostudies-arrayexpress
Project description:In order to find the miRNAs, target genes and signaling pathways related to the dwarf phenotype induced by NlCSP1, 35S-NlCSP1 and 35S-GFP control were infiltrated into Nicotiana benthamiana. We analyzed the differentially expressed miRNAs in the infiltrated leaves (NlCSP1_Z/GFP_Z) and the upper uninfiltrated leaves (NlCSP1_O/GFP_O) of N. benthamiana. A total of 106 and 97 differentially expressed miRNAs were identified in NlCSP1_Z/GFP_Z and NlCSP1_O/GFP_O, respectively. KEGG annotation revealed a large number of differentially expressed miRNAs involved in plant hormone signal transduction, indicating that NlCSP1 changed the hormone signal pathway in N. benthamiana.
2021-09-01 | GSE169282 | GEO
Project description:Chemotyping dataset of n-butanol peptide extract of Nicotiana benthamiana leaves six days after infiltration with Agrobacterium tumefaciens LBA4404 pEAQ-HT
2018-06-29 | MSV000082533 | MassIVE
Project description:Chemotyping of n-butanol peptide extract of Nicotiana benthamiana leaves six days after infiltration with Agrobacterium tumefaciens LBA4404 pEAQ-HT-CanBURP
2018-06-29 | MSV000082535 | MassIVE
Project description:Chemotyping dataset of n-butanol peptide extract of Nicotiana benthamiana leaves six days after infiltration with Agrobacterium tumefaciens LBA4404 pEAQ-HT-LbaLycA
2018-06-29 | MSV000082536 | MassIVE
Project description:Chemotyping dataset of n-butanol peptide extract of Nicotiana benthamiana leaves six days after infiltration with Agrobacterium tumefaciens LBA4404 pEAQ-HT-StuBURP
2018-06-29 | MSV000082557 | MassIVE
Project description:This dataset belongs to a set of three RNA-Seq experiments that were carried out to study the regulation of monoterpenoid indole alkaloid production in the medicinal plant Catharanthus roseus. For this dataset, C. roseus flower petals were infiltrated with Agrobacterium tumefaciens C58C1 or infiltration buffer as control. For each sample, flower petals from four to five flowers, each from a different individual plant were infiltrated.
2020-09-07 | E-MTAB-9517 | biostudies-arrayexpress
Project description:Chemotyping dataset of n-butanol peptide extract of Nicotiana benthamiana leaves six days after infiltration with Agrobacterium tumefaciens LBA4404 pEAQ-HT-Sali3-2-[QPAGVYTW]
2018-06-29 | MSV000082539 | MassIVE
Project description:Chemotyping dataset of n-butanol peptide extract of Nicotiana benthamiana leaves six days after infiltration with Agrobacterium tumefaciens LBA4404 pEAQ-HT-Sali3-2-[QPWGVGAW]
2018-06-29 | MSV000082542 | MassIVE