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Transcriptional profiles of human CD14+ monocytes conditioned with melanoma exosomes

ABSTRACT: Transcriptional profiles of human CD14+ monocytes conditioned with melanoma exosomes

PROVIDER: PRJNA396196 | ENA |

REPOSITORIES: ENA

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Transcriptional profiles of human CD14+ monocytes conditioned with melanoma exosomes [miRNA expression]

Project description:We analyzed gene and microRNA expression profiles of purified CD14 monocytes isolated from blood of 5 healthy donors after 24 h of in vitro co-culture with exosomes exosomes isolated from the INT12 melanoma cell line generated in our laboratory from a human melanoma specimen. Differentially expressed genes and miRNAs were identified between treated and untreated monocytes.

2018-10-26 | GSE102010 | GEO

Transcriptional profiles of human CD14+ monocytes conditioned with melanoma exosomes [gene expression]

2018-10-26 | GSE102009 | GEO

RNA profiling of human lymphatic endothelial cells exposed to melanoma exosomes

Project description:In order to study the influence of melanoma-derived exosomes in the lymphatic vasculature within the lymph nodes, we have profile the transcriptional changes occurring in human lymphatic endothelial cells treated with melanoma-secreted exosomes or with the conditioned medium from melanoma cells depleted of exosomes.

2021-07-16 | GSE135187 | GEO

Gene expression profiles of human blood classical monocytes (CD14++CD16-), CD16 positive monocytes (CD14+16++ and CD14++CD16+), and CD1c+ CD19- dendritic cells [human data]

Project description:In this study gene expression of human blood classical monocytes (CD14++CD16-), CD16 positive monocytes (consisting of non-classical CD14+16++ and intermediate CD14++CD16+ monocytes) and CD1c+ CD19- dendritic cells from healthy subjects were investigated. Keywords: expression profiling by array

2011-12-17 | GSE34515 | GEO

Transcriptional profiles of human CD14+ monocytes conditioned with melanoma exosomes

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-10-26 | GSE102011 | GEO

Transcription profiling by array of human serum-free fibrocytes, serum-containing fibrocytes, CD14++CD16- Monocytes, CD14++CD16+ Monocytes, CD14+CD16++ Monocytes and macrophages

Project description:Serum-free Fibrocytes, Serum-containing Fibrocytes, CD14++CD16- Monocytes, CD14++CD16+ Monocytes, CD14+CD16++ Monocytes, Macrophages were all generated from up to 3 biological replicates from each of 3 separate donors. RNA was extracted (Ambion RNAqueous), labelled with cy3, mixed with cy5 labelled human reference (Stratagene), and hybridised to slides printed with Human AROS v4.0 oligonucleotides (Operon). Slides were scanned using a Perkin Elmer GX plus, and the data then normalised with GEPAS v4.0 and collated. Final data analysis was carried out using TMEV 4.0. SAM was performed using a 0.1% FDR. PCA were plotted from this list, and interrogation carried out using DAVID to determine pathway enrichment.

2011-01-01 | E-MEXP-2811 | biostudies-arrayexpress

Homo sapiens

Project description:Transcriptional profiles of human CD14+ monocytes conditioned with melanoma exosomes [miRNA expression]

| PRJNA396200 | ENA

Homo sapiens

Project description:Transcriptional profiles of human CD14+ monocytes conditioned with melanoma exosomes [gene expression]

| PRJNA396201 | ENA

SuperSAGE evidence for CD14++CD16+ monocytes as a third monocyte subset

Project description:Monocytes are a heterogeneous cell population with subset-specific functions and phenotypes. The differential expression of CD14 and CD16 distinguishes classical CD14++CD16-, intermediate CD14++CD16+ and non-classical CD14+CD16++ monocytes. However, CD14++CD16+ monocytes remain the most poorly characterized subset so far. Therefore we analyzed the transcriptomes of the three monocyte subsets using SuperSAGE in combination with high-throughput sequencing. Analysis of 5,487,603 tags revealed unique identifiers of CD14++CD16+ monocytes, delineating these cells from the two other monocyte subsets. CD14++CD16+ monocytes were linked to antigen processing and presentation (e.g. CD74, HLA-DR, IFI30, CTSB), to inflammation and monocyte activation (e.g. TGFB1, AIF1, PTPN6), and to angiogenesis (e.g. TIE2, CD105). Therefore we provide genetic evidence for a distinct role of CD14++CD16+ monocytes in human immunity. Human monocyte subsets (CD14++CD16-, CD14++CD16+, CD14+CD16++) were isolated from 12 healthy volunteers based on MACS technology. Total RNA from monocyte subsets was isolated and same aliquots from each donor and monocyte subset were matched for SuperSAGE. Three SuperSAGE libraries (CD14++CD16-, CD14++CD16+ and CD14+CD16++) were generated.

2011-07-24 | E-GEOD-30811 | biostudies-arrayexpress

SuperSAGE evidence for CD14++CD16+ monocytes as a third monocyte subset

2011-07-24 | GSE30811 | GEO

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