Project description:Monastrol treatment of Leishmania donovani infected macrophages Macrophages were infected with Leishmania donovani and treated with monastrol to look for signalling molecules
Project description:The mRNA expression of antimony resistant strains of Leishmania donovani was compared to the expression of the sensitive Leishmania donovani.
Project description:The mRNA expression of antimony resistant strains of Leishmania donovani was compared to the expression of the sensitive Leishmania donovani. The antimony resistant and sensitive Leishmania donovani were grown in complete M199 medium with 10% FCS and Penicillin streptomycin mixture. At stationary phase (5 day culture) cells were harvested in sterile Phosphate buffered saline and used for RNA isolation.
Project description:Differential gene expression analysis was carried out in clinical isolates of Leishmania donovani (L. donovani) parasite derived from VL patient before miltefosine treatment, post treatment and patient that relpased after miltefosine treatment
Project description:Monocyte derived dendritic cells (MDDC) were infected with Leishmania major or Leishmania donovani parasites and collected at 4, 8, and 24 hours post-infection to analyze the differential effects those parasite species have on human host cell gene expression over time.
