Human Sputum
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA397083 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Comparison of the gene expression profile of Moraxella catarrhalis grown in the presence of 20% pooled human sputum in chemically-defined medium relative to Moraxella catarrhalis grown in chemically-defined medium alone.
2014-02-02 | GSE27059 | GEO
Project description:Comparison of the gene expression profile of Moraxella catarrhalis grown in the presence of 20% pooled human sputum in chemically-defined medium relative to Moraxella catarrhalis grown in chemically-defined medium alone. Moraxella catarrhalis ATCC43617 was grown to mid-logarithmic phase either in the presence of 20% pooled human sputum in chemically-defined medium or in chemically-defined medium alone. Total RNA was extracted from bacterial cells exposed to each of these conditions and cDNA was generated for CyDye labelling. 3 biologic replicates were generated and each replicate underwent a dye swap (total of 6 experimental data collections). The gene expression profile reported is that of Moraxella catarrhalis grown in the presence of pooled human sputum in a chemically-defined medium relative to Moraxella catarrhalis grown only in the presence of the chemically-defined medium.
2014-02-02 | E-GEOD-27059 | biostudies-arrayexpress
Project description:Chronic respiratory diseases are a growing burden, and potentially life-threatening condition if left untreated. It is an urgent medical concern that requires proper diagnosis, treatment, and management to prevent serious complications. Analyzing induced sputum is a non-invasive approach for investigating the mechanisms and progression of respiratory diseases. It allows for the collection of sputum samples without disrupting the patient's normal life. By analyzing the proteins and other molecular substances present in the sputum, it can provide valuable insights into the pathological changes and evaluate the effectiveness of treatments for respiratory diseases. In this study, we carried out proteomic analysis of three types of sputum collected from patients with chronic respiratory diseases using three different digestion methods. In comparison, FASP method was found to be more efficient than In-gel digestion and in-solution digestions, yielding the highest number of unique protein identifications. Integrate the proteomic and phosphoproteomic profiling of three types of sputum, it was observed that raw sputum samples exhibited a higher number of unique protein and phosphoprotein identifications. Our study provides valuable insights into the application of proteomics to the analysis of sputum samples, and serves as a useful resource for understanding the potential of this approach in the study of respiratory diseases.
2023-01-28 | PXD039725 | iProX
Project description:Sputum cells collected before (visit 2) and after (visit 4) allergen challenge in asthma patients were isolated and RNA purified for analysis on gene expression arrays. Human subject recruitment part of NIH sponsored protocol as part of the Eosinophil Program Project Grant (PI: Dr. Nizar Jarjour) Sputum cell RNA collected from induced sputum cells before and 48 hours after whole-lung allergen challenge.
2013-04-20 | E-GEOD-46238 | biostudies-arrayexpress
Project description:Gene expression profiling was performed on sputum samples obtained from asthmatics and matched healthy controls, to identify markers associated with various asthma subtypes. Sputum samples were collected from asthmatics and healthy controls and subjected to expression profiling using Affymetrix HG-U133Plus2.0 microarrays.
2015-10-23 | E-GEOD-41863 | biostudies-arrayexpress
Project description:We evaluated the applicability and usability of whole-genome methylomics of sputum samples in molecular profiling of chronic inflammatory lung diseases. Genomic DNA was purified from sputum samples of subjects with Asthma, COPD as well as healthy controls and analyzed on the Illumina Infinium HumanMethylation 450k platform.
2020-10-25 | GSE148000 | GEO
Project description:Gene expression profiling was performed on sputum samples obtained from asthmatics and matched healthy controls, to identify markers associated with various asthma subtypes.
2015-10-23 | GSE41863 | GEO
Project description:Sputum expectorated by the pulmonary tuberculosis patients still remains a primary diagnostic specimen. The expression pattern of mycobacteria in sputum will lead to an insight of bacterial adaptation at the most highly transmissible stage of infection and can also help in identifying newer diagnostic as well as drug targets. Further, the identification of crossreactive gene targets from the sputum samples of other lung diseases will help to find the diagnostic candidates which will be highly specific to pulmonary tuberculosis.
2017-04-18 | GSE93316 | GEO
Project description:The study aimed to compare the gene expression profiles at a single cell level in Sputum cells between patients with cystic fibrosis (CF) and disease controls (CTRL).
2020-07-01 | GSE145360 | GEO